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Biological Characterization and Pluripotent Identification of Cartilage Stem/Progenitor from Peking Duck

文献类型： 外文期刊

作者： Liu, Xibin 1 ; Guan, Weijun 2 ; Zheng, Dong 1 ;

作者机构： 1.Northeast Forestry Univ, Coll Wildlife & Protected Area, Harbin 150040, Peoples R China

2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

3.Yantai Univ, Grad Management Div, Student Affairs Off, Yantai 264005, Peoples R China

关键词：   Peking duck; Cartilage stem; progenitor; Biological characterization; Directional differentiation

期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.6； 五年影响因子：0.7 ）

ISSN： 0030-9923

年卷期： 2023 年 55 卷 1 期

页码：

收录情况： SCI

摘要： A group of cells in cartilage tissue with stem cell characteristics may be ideal seed cells for cartilage defect repair. In previous studies, stem/progenitor cells from articular cartilage have been isolated and identified in humans, horses, and bovines, but rarely in ducks. We isolated and cultured cartilage stem/progenitor cells (CPSCs) from the articular cartilage of 14-day-old Peking duck embryos using the fibronectin adhesion method. The morphology of CPSCs was observed under an inverted microscope. Based on the growth curve test, we found that the growth mode of the cells was "S" type growth. The clonogenic assay was performed to determine the clonogenic efficiency CPSCs. The expression of cell surface markers was detected by immunofluorescence, flow cytometry, and RT-PCR, and it was found that the CPSCs positively expressed FGFR-3, collagen type II, CD44, CD73, CD90, vimentin, Notch1, CD105, and CD106, but negatively expressed the haematopoietic stem cell surface marker CD34. Furthermore, CPSCs were successfully induced to differentiate into adipocytes, osteoblasts, and chondrocytes, showing that they have an in vitro differentiation ability. These findings provide a theoretical and experimental basis for cell transplantation therapy in tissue engineering.

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