In Vitro Culture and Multipotency Evaluation of Broiler Umbilical Cord Mesenchymal Stem Cells
文献类型： 外文期刊
作者： Ma, Caiyun 1 ; Liu, Gaofeng 1 ; Wang, Chunjing 1 ; Guo, Yu 1 ; Guan, Weijun 2 ; Liu, Changqing 1 ; Wen, Hebao 1 ;
作者机构： 1.Bengbu Med Coll, Sch Life Sci, Bengbu, Anhui, Peoples R China
2.Chinese Acad Agr Sci, Inst Beijing Anim Sci & Vet, Beijing, Peoples R China
关键词： UMSCs; broiler; biological characteristics; multilineage potential; regenerative therapies
期刊名称：BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY （ 影响因子：1.0； 五年影响因子：1.1 ）
ISSN： 1516-8913
年卷期： 2023 年 66 卷
页码：
收录情况： SCI
摘要： Umbilical cord mesenchymal stem cells (UMSCs) from fetal tissues have become a valuable source of cell regeneration therapy due to their low immunogenicity, high plasticity and antimicrobial peptides. Most researches about UMSCs mainly focused on humans, mice, rabbits and other mammals, but it is still unknown in broiler. To the best of our knowledge, we first established a broiler-derived UMSCs line from noncontroversial Wharton's jelly of umbilical cord. The detection of biological characteristics showed UMSCs showed the morphological characteristics of fibroblasts and could be cultured to 26 generations in vitro, and maintained diploid chromosomes (2n=78) by G-banding analysis. Cell cycle of UMSCs showed the rate of G0/G1 was about 76.7-80.9%. Moreover, UMSCs could express mesenchymal markers (CD29, CD90, CD71, and CD44), but not hematopoietic marker (CD34) and endothelial marker (CD31). Additionally, UMSCs could be efficiently induced to transdifferentiate into three germ layer cells, such as adipocytes, osteoblasts, chondrocytes, hepatocytes, epithelia, and neurogenic cells. These results demonstrated UMSCs have the essential characteristics of MSCs, and can be used as an attractive candidate for cell regenerative therapy and the study of regulatory mechanisms associated with selective differentiation.
- 相关文献
作者其他论文 更多>>
-
Analysis of Flavor Differences in Yak Milk Powder at Different Milk Production Stages by Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry
作者：Wang, Diandian;Yan, Wenjie;Wang, Diandian;Zhao, Jian;Guo, Yu;Yan, Wenjie;Zhou, Yaxi
关键词：yak milk; volatile compounds; HS-SPME-GC-MS; OPLS-DA; hierarchical clustering
-
Differentiation and characterization of volatile compounds in five common milk powders using HS-GC-IMS, HS-SPME-GC-MS, and multivariate statistical approaches
作者：Zhou, Yaxi;Wang, Diandian;Yan, Wenjie;Zhou, Yaxi;Wang, Diandian;Zhao, Jian;Guo, Yu;Yan, Wenjie;Zhou, Yaxi
关键词：Milk powder; Volatile compounds; HS-GC-IMS; HS-SPME-GC-MS; OPLS-DA
-
Exploring the link between M1 macrophages and EMT of amniotic epithelial cells: implications for premature rupture of membranes
作者：Gao, Yuhua;Miao, Wang;Zhang, Jingmiao;Liu, Yize;Li, Zhikun;Song, Jiaxun;Bai, Chunyu;Zhang, Yanan;Mi, Ningning;Li, Xiangchen;Gao, Yuhua;Guan, Weijun;Bai, Chunyu
关键词：Amniotic epithelial cells; Extracellular vesicles; Macrophages; Epithelial-mesenchymal transition
-
Correlation of Midgut Microbiota and Metabolic Syndrome-Related Lipids in Hemolymph Between Obese and Lean Silkworm Strains
作者：Guo, Huiduo;Wang, Yalei;Guo, Yu;Liu, Xiangbiao;Gui, Tao;Ling, Mingfa;Qian, Heying;Guo, Huiduo;Wang, Yalei;Guo, Yu;Liu, Xiangbiao;Gui, Tao;Ling, Mingfa;Qian, Heying
关键词：silkworm; metabolic syndrome; hemolymph; lipids; midgut microbiota
-
The Seasonal Response of N2O Emissions to Increasing Precipitation and Nitrogen Deposition and Its Driving Factors in Temperate Semi-Arid Grassland
作者：Peng, Qin;Qi, Yuchun;Guo, Yu;Dong, Yunshe;Yuan, Xiujin;Yin, Feihu;Lv, Ning;Yin, Feihu;Lv, Ning;Liu, Xingren;Yuan, Xiujin
关键词：seasonal N2O emissions; water addition; N deposition; temperate grassland
-
A telomere-to-telomere genome assembly of Zhonghuang 13, a widely-grown soybean variety from the original center of Glycine max
作者：Zhang, Anqi;Kong, Tangchao;Qiu, Shizheng;Guo, Jiahe;Ruan, Shuyong;Guo, Yu;Guo, Jirui;Zhang, Zhishuai;Liu, Yue;Hu, Zheng;Jiang, Tao;Liu, Yadong;Cao, Shuqi;Hu, Yang;Liu, Bo;Wang, Yadong;Sun, Baiquan;Sun, Shi;Wu, Tingting;Jiang, Bingjun;Yang, Maoxiang;Yao, Xiangyu;Han, Tianfu;Hong, Huilong
关键词：Soybean; Telomere-to-Telomere assembly; Zhonghuang 13; Structure variations
-
Most Tibetan weedy barleys originated via recombination between Btr1 and Btr2 in domesticated barley
作者：Gao, Guangqi;Yan, Luxi;Cai, Yu;Jiang, Congcong;He, Qiang;Tasnim, Sarah;Liu, Jun;Zhang, Jing;Yang, Ping;Yan, Luxi;Cai, Yu;Feng, Zongyun;Guo, Yu;Mascher, Martin;Komatsuda, Takao
关键词：Tibetan weedy barley; agriocrithon; de-domestication; out-pollination; recombination; brittle rachis
