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Lead induces mouse skin fibroblast apoptosis by disrupting intracellular homeostasis

文献类型： 外文期刊

作者： Wang, Hui 1 ; Wang, Huinuan 1 ; Guan, Jiawen 1 ; Guan, Weijun 3 ; Liu, Zheng 1 ;

作者机构： 1.Jinzhou Med Univ, Jinzhou 121001, Peoples R China

2.Meat Proc & Safety Control Engn Technol Res Ctr Li, Jinzhou 121001, Peoples R China

3.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.6； 五年影响因子：4.9 ）

ISSN： 2045-2322

年卷期： 2023 年 13 卷 1 期

页码：

收录情况： SCI

摘要： Lead (Pb) is a critical industrial and environmental contaminant that can cause pathophysiological changes in several cellular and organ systems and their processes, including cell proliferation, differentiation, apoptosis, and survival. The skin is readily exposed to and damaged by Pb, but the mechanisms through which Pb damages cells are not fully understood. We examined the apoptotic properties of Pb in mouse skin fibroblast (MSF) in vitro. Treatment of fibroblasts with 40, 80, and 160 mu M Pb for 24 h revealed morphological alterations, DNA damage, enhanced caspase-3, -8, and -9 activities, and apoptotic cell population. Furthermore, apoptosis was dosage (0-160 mu M) and time (12-48 h) dependent. Concentrations of intracellular calcium (Ca2+) and reactive oxygen species were increased, and the mitochondrial membrane potential was decreased in exposed cells. Cell cycle arrest was evident at the G0/G1 phase. The Bax, Fas, caspase-3 and -8, and p53 transcript levels were increased, whereas Bcl-2 gene expression was decreased. Based on our analysis, Pb triggers MSF apoptosis bydisrupting intracellular homeostasis. Our findings enrich the knowledge about the mechanistic function of Pb-induced cytotoxicity on human skin fibroblasts and could potentially guide future Pb health risk assessments.

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