Rapid and direct detection of m6A methylation by DNAzyme-based and smartphone-assisted electrochemical biosensor
文献类型： 外文期刊
作者： Li, Kai 1 ; Liang, Yu 1 ; Li, Xinran 1 ; Yang, Mengrui 1 ; Wang, Min 1 ; Li, Fukai 1 ; Qi, Xin 1 ; Zhou, Jian 1 ; Fu, Wei 2 ; Li, Liang 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China
2.Minist Agr & Rural Affairs, Dev Ctr Sci & Technol, Beijing 100176, Peoples R China
关键词： DNAzyme; Tetrahedron DNA; Electrochemical biosensor; m(6)A methylation
期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.5； 五年影响因子：10.1 ）
ISSN： 0956-5663
年卷期： 2025 年 267 卷
页码：
收录情况： SCI
摘要： m(6)A methylation detection is crucial for understanding RNA functions, revealing disease mechanisms, guiding drug development and advancing epigenetics research. Nevertheless, high-throughput sequencing and liquid chromatography-based traditional methods still face challenges to rapid and direct detection of m(6)A methylation. Here we report a DNAzyme-based and smartphone-assisted electrochemical biosensor for rapid detection of m(6)A. We initially identified m(6)A methylation-sensitive DNAzyme mutants through site mutation screening. These mutants were then combined with tetrahedral DNA to modify the electrodes, creating a 3D sensing interface. The detection of m(6)A was accomplished by using DNAzyme to capture and cleave the m(6)A sequence. The electrochemical biosensor detected the m(6)A sequence at nanomolar concentrations with a low detection limit of 0.69 nM and a wide detection range from 10 to 10(4) nM within 60 min. As a proof of concept, the 3 '-UTR sequence of rice was selected as the m(6)A analyte. Combined with a smartphone, our biosensor shows good specificity, sensitivity, and easy-to-perform features, which indicates great prospects in the field of RNA modification detection and epigenetic analysis.
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