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Bmo-miR-2780a regulates the expression of the sericin-1 gene of Bombyx mori

文献类型： 外文期刊

作者： Ping Qian 1 ; Xin Wang 3 ; Li, Jiashuang 1 ; Tao Jiang 1 ; Tang, Xudong 1 ; Guan Huixiang 1 ; Shen, Xingjia 1 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural Biol & Biotechnol, Zhenjiang 212003, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Res Inst, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr, Zhenjiang, Jiangsu, Peoples R China

3.Silkworm Eggs Adm Dept Jiangsu Prov, Wuxi, Jiangsu, Peoples R China

4.Univ Haifa, Fac Nat Sci, Inst Evolut, Dept Evolutionary & Environm Biol, Haifa, Israel

关键词： bmo-miR-2780a; Bombyx mori; posttranscriptional regulation; sericin-1 gene

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

ISSN： 0739-4462

年卷期： 2020 年 103 卷 2 期

页码：

收录情况： SCI

摘要： Silk production in Bombyx mori L. is largely determined by the expression of genes encoding fibroin and sericin. Here, we examined the regulatory function of a microRNA (miRNA) on silk gene expression using the sericin-1 gene (BmSer-1). First, we downloaded whole mature miRNAs of silkworm from miRBase and identified bmo-miR-2780a as a candidate miRNA for the regulation of BmSer-1 expression. We used semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) with stem-loop primers to investigate the expression profile of bmo-miR-2780a and its predicted target gene BmSer-1 in seven different tissues from 5th instar day-3 larvae, including head, fat body, anterior silk gland (ASG), middle silk gland (MSG), posterior silk gland (PSG), middle gut, and hemolymph. Our results showed that bmo-miR-2780a was specifically expressed in the MSG and that the expression level of BmSer-1 was significantly higher in the MSG than in other tissues. Recombinant plasmids carrying both pri-mir-2780a and Ser1-3 ' UTR were constructed and then used to cotransfect BmN cells. We further detected the effect of bmo-miR-2780a on Ser-1 in vivo. These results showed that the target gene was significantly decreased by miR-2780a compared with the control group (p<.05), thus indicating that bmo-miR-2780a might negatively regulate the expression of Ser-1.

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