Construction and immunogenicity analysis of a recombinant baculovirus targeting the N protein of SARS-CoV-2
文献类型： 外文期刊
作者： Yu, Bohan 1 ; Mo, Xiaoli 1 ; Sui, Li 1 ; Fang, Yujia 1 ; Tang, Xudong 1 ; Qian, Ping 1 ;
作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural & Anim Biotechnol, Zhenjiang 212100, Peoples R China
2.Chinese Acad Agr Sci, Sericultural Sci Res Ctr, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr & Rural Affairs, Zhenjiang 212100, Peoples R China
关键词： Baculovirus; COVID-19 N protein; Immunogenicity protein expression; Sodium butyrate
期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.1； 五年影响因子：2.5 ）
ISSN： 0141-5492
年卷期： 2025 年 47 卷 3 期
页码：
收录情况： SCI
摘要： ObjectivesThis study aimed to construct recombinant baculoviruses capable of expressing the nucleocapsid N protein of SARS-CoV-2 and to assess the effects of co-administration with sodium butyrate on its expression and immunogenicity.ResultsThe recombinant BmNPV expressed green fluorescent protein in BmN cells and N protein in mammalian cells. The addition of sodium butyrate significantly enhanced the expression of N protein in HEK293T cells. Following intramuscular injection of recombinant BmNPV into mice, specific antibodies against the N protein were detectedon day 7, 21, and 35. Co-administration with sodium butyrate (1000 mg/kg body weight) significantly enhanced the immunogenicity of the recombinant virus.ConclusionsRecombinant BmNPV expressing the SARS-CoV2 N protein successfully inducedthe anti-N immunogenicity in mice, providing a solid foundationfor the development of novel subunit vaccines against SARS-CoV-2.
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