您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Development of a stable luciferase@nanoflower-based method for the sensitive detection of pesticide residues in milk

文献类型： 外文期刊

作者： Li, Xiaoyu 1 ; Huang, Fengchun 1 ; Zhang, Juan 1 ; Chen, Jiaci 1 ; Pang, Xiangyi 1 ; Yang, Longrui 1 ; Sun, Xiaoyun 1 ; Fan, Yuhang 1 ; Chen, Ailiang 1 ; Yang, Qingli 2 ;

作者机构： 1.Chinese Acad Agr Sci, Inst State Key Lab Qual & Safety Agroprod, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China

2.Qingdao Agr Univ, Inst Food Sci & Engn, Qingdao 266109, Shandong, Peoples R China

关键词： Luciferase; Nanoflowers; CaCl2; Bioluminescence; Pesticide residue detection

期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）

ISSN： 0308-8146

年卷期： 2025 年 483 卷

页码：

收录情况： SCI

摘要： Acetylcholinesterase inhibition-luciferase bioluminescence system enables rapid detection of organophosphate and carbamate pesticide residues. However, firefly luciferase (FLuc) suffers from poor stability and short luminescence duration, thereby introducing uncertainties in detection. To address this problem, nanoflowerimmobilized FLuc (FLuc@NFs) was prepared using a coprecipitation method, which markedly enhanced stability and activity. FLuc@NFs exhibited intact nanoflower structures and allowed storage at 25 degrees C and at 4 degrees C for 2 weeks, overcoming the limitation of free enzyme storage at-20 degrees C. In the bioluminescence reaction, FLuc@NFs at 1 mg/mL achieved 62.14 % higher luminescence than free FLuc, with stable signals extending from 10 s to 30 min, enabling high-throughput detection. The system achieved detection limits of 5 and 10 ng/mL for chlorpyrifos and carbaryl, respectively, demonstrating improved reliability and sensitivity in pesticide detection.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台