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Construction of an infectious full-length cDNA clone of potato aucuba mosaic virus

文献类型: 外文期刊

作者: Chen, Buyang 1 ; Lin, Qi 1 ; Yin, Yueyan 1 ; Jiang, Liangliang 1 ; Wang, Fang 3 ; Hu, Shuzhen 1 ; Wu, Xinyang 1 ; Xu, Fei 5 ;

作者机构: 1.Ningbo Univ, Inst Plant Virol, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China

2.Yunnan Acad Agr Sci, Inst Alpine Econ Plant, Lijiang 674100, Yunnan, Peoples R China

3.Ningbo Acad Agr Sci, Ningbo 315211, Peoples R China

4.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China

5.Zhejiang Acad Agr Sci, Cent Lab, Hangzhou 310021, Peoples R China

6.Yunnan Acad Tobacco Agr Sci, Kunming 650021, Yunnan, Peoples R China

期刊名称:ARCHIVES OF VIROLOGY ( 影响因子:2.574; 五年影响因子:2.466 )

ISSN: 0304-8608

年卷期: 2021 年 166 卷 5 期

页码:

收录情况: SCI

摘要: Potato aucuba mosaic virus (PAMV), a positive single-strand RNA virus, has one of the longest genomes of the viruses in the genus Potexvirus. In 2019, potato samples with mottle and crinkling symptoms from Huzhou, Zhejiang province, China, were identified to be infected with PAMV, potato virus X (PVX), and potato virus Y (PVY) by transcriptome sequencing. To study the effects of single infection by PAMV, the full-length sequence of PAMV from Huzhou (MT193476) was determined and an infectious full-length cDNA clone was constructed. This cDNA clone was infectious by agro-infiltration, leading to systemic symptoms in Nicotiana benthamiana, tomato, pepper, and potato.

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