Construction of chimeric viruses based on pepper mild mottle virus using a modified Cre/loxP system
文献类型： 外文期刊
作者： Yin, Yue-yan 1 ; Hua, Meng-ying 3 ; Zhao, Kuang-jie 3 ; Wan, Qiong-lian 1 ; Bu, Shan 4 ; Lu, Yu-wen 3 ; Zheng, Hong-ying 3 ; Rao, Shao-fei 3 ; Yan, Fei 3 ; Peng, Jie-jun 3 ; Chen, Hai-ru 1 ; Chen, Jian-ping 1 ;
作者机构： 1.Yunnan Agr Univ, Coll Plant Protect, Kunming 650201, Peoples R China
2.Yunnan Acad Agr Sci, Inst Alpine Econ Plants, Lijiang 674100, Peoples R China
3.Ningbo Univ, Inst Plant Virol, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China
4.Hunan Univ, Longping Branch Grad Coll, Changsha 410125, Peoples R China
关键词： pepper mild mottle virus; Cre; loxP; necrosis; infectious cDNA clone; chimeric virus
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.384； 五年影响因子：4.021 ）
ISSN： 2095-3119
年卷期： 2022 年 21 卷 8 期
页码：
收录情况： SCI
摘要： Cre/loxP, a site-specific recombination system, has been widely used for various purposes, including chromosomal translocations, generation of marker-free transgenic plants, tissue-specific activation of a reporter gene and efficient heterologous gene expression in plants. However, stable or transient expression of Cre recombinase in plants can cause chlorosis or necrosis. Here, we describe a modified Cre/loxP recombination system using a DNA fragment flanked with loxP sites in the same orientation in which necrosis induced by Cre recombinase in Nicotiana benthamiana leaves was alleviated. The modified system was successfully used to create functional GFP-tagged pepper mild mottle virus (PMMoV) and a chimeric virus with coat protein (CP) substitution assembled from separate pro-vector modules. Our results provide a new strategy and flexible technique to construct chimeric virus and infectious clones for plant viruses with large genomes.
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