文献类型： 外文期刊
作者： Liu, Q. 1 ; Qiu, S. 1 ; Xu, Z. 1 ; Wang, X. 1 ; Shen, H. 3 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab Agrofood Safety & Qual, Beijing 100081, Peoples R China
3.Beijing Univ Agr, Coll Anim Sci & Technol, Beijing 102206, Peoples R China
关键词： deoxynivalenol; cytotoxicity; human primary hepatocytes; metabolomics
期刊名称：WORLD MYCOTOXIN JOURNAL （ 影响因子：3.353； 五年影响因子：3.108 ）
ISSN： 1875-0710
年卷期： 2020 年 13 卷 4 期
页码：
收录情况： SCI
摘要： To investigate the cytotoxicity of deoxynivalenol (DON) on human embryo liver CCC-HEL-1 and hepatoma cell line HepG2 cell models, both cell experience and metabolomic approach were studied. For the cell evaluation, cells viabilities of CCC-HEL-1 and HepG2 were decreased in both a time- and dose-dependent manner at concentration range from 0.08 similar to 10 mu mol/l, after which the concentration of 1 mu mol/l DON was selected for the next experiments. A higher production of reactive oxygen species (ROS) in DON treated CCC-HEL-1 cells was found after 2 h treatment compared with the HepG2 group, while ROS generation was significantly dropped after 48 h in both models. DON-treated CCC-HEL-1 and HepG2 cells displayed significantly decreased percentages of Delta Psi m loss. For the metabolomic study based on liquid chromatography quadrupole time-of-flight mass spectrometry, it was notable that certain amino acids identified in the two DON-treated groups were upregulated. The pathway analysis also revealed that amino acid metabolism played a crucial role underlying DON exposure in the two studied models. Our results provided metabolic evidence that further confirmed the toxicological potential of DON to disturb amino acid and lipid metabolism in human embryo liver cells.
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