Characterization of a novel cold-adapted GH1 β-glucosidase from Psychrobacillus glaciei and its application in the hydrolysis of soybean isoflavone glycosides
文献类型： 外文期刊
作者： He, Jinjian 1 ; Duan, Jiajing 1 ; Yu, Pinglian 2 ; Li, Yuying 1 ; Wang, Mansheng 1 ; Zhang, Xiu 5 ; Chen, Zishu 1 ; Shi, Pengjun 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Peoples R China
2.Zhaotong Univ, Yunnan Univ Plateau Characterist Funct Food, Sch Chem & Chem Engn, Key Lab, Zhaotong 657000, Peoples R China
3.Tianjin Agr Univ, Coll Hort & Landscape, Tianjin 300392, Peoples R China
4.Chinese Acad Agr Sci, Inst Food Sci & Technol, Beijing 100081, Peoples R China
5.North Minzu Univ, Coll Biol Sci & Engn, Ningxia Key Lab Dev & Applicat Microbial Resources, Yinchuan 750021, Peoples R China
关键词： Psychrobacillus glaciei; beta-Glucosidase; Cold adaptation; Sugar tolerance; Catalytic efficiency; Isoflavone glycosides
期刊名称：CURRENT RESEARCH IN FOOD SCIENCE （ 影响因子：6.2； 五年影响因子：6.2 ）
ISSN：
年卷期： 2024 年 8 卷
页码：
收录情况： SCI
摘要： The novel beta-glucosidase gene ( pgbgl1 ) of glycoside hydrolase (GH) family 1 from the psychrotrophic bacterium Psychrobacillus glaciei sp. PB01 was successfully expressed in Escherichia coli BL21 (DE3). The deduced PgBgl1 contained 447 amino acid residues with a calculated molecular mass of 51.4 kDa. PgBgl1 showed its maximum activity at pH 7.0 and 40 degrees C, and still retained over 10% activity at 0 degrees C, suggesting that the recombinant PgBgl1 is a cold -adapted enzyme. The substrate specificity, K m , V max , and K cat / K m for the p -Nitrophenyl-beta-D-glucopyr- anoside ( p NPG) as the substrate were 1063.89 U/mg, 0.36 mM, 1208.31 U/mg and 3871.92/s, respectively. Furthermore, PgBgl1 demonstrated remarkable stimulation of monosaccharides such as glucose, xylose, and galactose, as well as NaCl. PgBgl1 also demonstrated a high capacity to convert the primary soybean isoflavone glycosides (daidzin, genistin, and glycitin) into their respective aglycones. Overall, PgBgl1 exhibited high catalytic activity towards aryl glycosides, suggesting promising application prospects in the food, animal feed, and pharmaceutical industries.
- 相关文献
作者其他论文 更多>>
-
Effects of N-linked glycosylation on the enzymatic properties of GH12 bifunctional enzymes from Aspergillus terreus expressed in Pichia pastoris
作者：Zuo, Dinghui;Xia, Shenju;Liu, Huiqin;Zuo, Dinghui;He, Jinjian;Sun, Xihang;Liu, Hanting;Xia, Shenju;Wang, Mansheng;Zheng, Xia;Shi, Pengjun
关键词：N -glycosylation; GH12 endoglucanase; Pichia pastoris; Thermalstability
-
Overexpression of TaSnRK2.9s Improves Plant Growth and Development in Rice (Oryza sativa L.)
作者：Rehman, Shoaib Ur;Zhang, Zijin;Chen, Jing;Rehman, Shoaib Ur;Khan, Zulqurnain;Waheed, Ummara;Hasnain, Muhammad Usama;Rehman, Shoaib Ur;Mao, Xinguo;Li, Yuying;Wang, Jingyi;Jing, Ruilian;Sajjad, Muhammad
关键词：cell membrane stability; common wheat; drought; gene expression; root development; transgenic rice
-
A Raf-Like MAPKKK Gene TaHT1 Controls Drought Tolerance and Primary Root Length in Wheat
作者：Wang, Min;Li, Chaonan;Wang, Jingyi;Li, Yuying;Zhang, Yanfei;Yang, Lili;Zhang, Yining;Zhang, Jie;Zhang, Zihui;Yan, Wen;Zuo, Yuang;Zhao, Qiancheng;Li, Long;Mao, Xinguo;Jing, Ruilian
关键词：drought tolerance; functional marker; primary root length;
TaHT1 ;TaSnRK2.10 -
Novel Thermostable Manganese Superoxide Dismutase from Alicyclobacillus sp. with High Specific Activity and Antioxidant Properties
作者：Sun, Xihang;Liu, Jinping;Ge, Qianqian;Jiang, Yayun;Xu, Yi;Li, Yuying;Hou, Zhenping;Shi, Pengjun;Liu, Jinping;Ge, Qianqian;Jiang, Yayun
关键词：superoxide dismutase; specific activity; thermostability; antioxidation; IPEC-J2 cell
-
The VP1 Protein of Porcine Teschovirus Inhibits the Innate Immune Response to Viral Infection by Blocking MDA5 Activation
作者：Li, Yuying;Zhang, Xinyu;Zhao, Baopeng;Zhao, Chenchen;Lei, Xiaoxiao;Huang, Haixin;Li, Chengkai;Lan, Tian;Sun, Wenchao;Zheng, Min;Lu, Huijun
关键词：
-
Porcine deltacoronavirus nsp5 antagonizes type I interferon signaling by cleaving IFIT3
作者：Huang, Haixin;Jin, Ningyi;Huang, Haixin;Lei, Xiaoxiao;Zhao, Chenchen;Qin, Yan;Li, Yuying;Zhang, Xinyu;Li, Chengkai;Lan, Tian;Zhao, Baopeng;Sun, Wenchao;Lu, Huijun;Jin, Ningyi
关键词：PDCoV; nsp5; IFIT3; cleavage; type I interferon signaling
-
Characterization of a Novel Hyperthermophilic GH1 β-Glucosidase from Acidilobus sp. and Its Application in the Hydrolysis of Soybean Isoflavone Glycosides
作者：He, Jinjian;Li, Yuying;Sun, Xihang;Zuo, Dinghui;Wang, Mansheng;Zheng, Xia;Shi, Pengjun;Zuo, Dinghui;Yu, Pinglian
关键词：beta-glucosidase; Acidilobus sp.; thermophilic; thermostability; ethanol tolerance; isoflavone glycosides
