The commercial PRRSV attenuated vaccine can be a potentially effective live trivalent vaccine vector
文献类型： 外文期刊
作者： Li, Yang 1 ; Wang, Yumiao 1 ; Pei, Xiuxiu 2 ; Wu, Yongshuai 1 ; Chen, Shao 1 ; Weng, Han 1 ; Jing, Yang 2 ; Ma, Zhiqian 1 ; Li, Zhiwei 1 ; Zheng, Zifang 1 ; Feng, Yingtong 2 ; Xu, Lele 1 ; Guo, Xuyang 1 ; Liu, Xiao 1 ; Zhang, Jianwu 1 ; Zheng, Haixue 1 ; Xiao, Shuqi 1 ;
作者机构： 1.Lanzhou Univ, Chinese Acad Agr Sci,Coll Vet Med, Lanzhou Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Lanzhou 730000, Peoples R China
2.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Peoples R China
关键词： PRRSV; Vaccine vector; Insertion; Marker gene; Multivalent vaccines; Infectious clone
期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.3； 五年影响因子：5.1 ）
ISSN： 0175-7598
年卷期： 2025 年 109 卷 1 期
页码：
收录情况： SCI
摘要： PRRSV is an immunosuppressive virus that is prone to secondary infection by other viral and microbial pathogens, exacerbating its harm to pigs. The development of multivalent vaccines that can prevent and control multiple pathogens is of great significance for the pig industry. In order to evaluate the potential of commercial PRRSV vaccines as the viral vectors for the trivalent vaccine, this study first embedded mCherry or EGFP genes for fluorescent proteins into the infectious clone of the HP-PRRSV MLV vaccine strain GD to construct a recombinant strain. Furthermore, we constructed a recombinant PRRSV strain, rPRRSV-mCherry-EGFP, that expresses both EGFP and mCherry proteins simultaneously and evaluated the stability and immunogenicity of the exogenous proteins. The results showed that MARC-145 cells infected with rPRRSV-mCherry-EGFP could simultaneously express both EGFP and mCherry exogenous proteins, and the number of EGFP and mCherry positive cells increased with virus infection, providing more fluorescent tool options for virus visualization and high-throughput screening of anti-PRRSV drugs. More importantly, the recombinant rPRRSV-mCherry-EGFP can be passaged in MARC-145 cells for at least 10 generations. It can induce piglets to produce antibodies against PRRSV, EGFP, and mCherry, indicating that the embedded two exogenous genes can also induce good immune responses. Our research suggests that using the infectious clone of a commercial HP-PRRSV-attenuated vaccine as a vector and embedding immune genes of two other pathogens to construct a recombinant strain may be an effective attempt to achieve cross-protection against three pathogens, providing an important research basis for the design of trivalent vaccines.
- 相关文献
作者其他论文 更多>>
-
Enhancing genetic transformation efficiency in cucurbit crops through AtGRF5 overexpression: Mechanistic insights and applications
作者：Li, Yang;Wang, Naonao;Feng, Jing;Liu, Yue;Wang, Huihui;Deng, Shijun;Dong, Wenjing;Liu, Xiaofeng;Lv, Bingsheng;Xu, Kuipeng;Zhang, Huimin;Zhang, Zhonghua;Chai, Sen;Sun, Jinjing
关键词：auxin; cucurbit crops; genetic transformation; GRF; regeneration; scRNA-Seq
-
Deciphering the flavor constituents, microbiota and physicochemical properties of Yancaigao, a traditional sour rice paste from Southwest China
作者：Gao, Jinxiao;Li, Yang;Zhang, Yue;Shan, Yimeng;Wang, Aixia;Zhang, Xiya;Wang, Fengzhong;Tong, Li-Tao;Gao, Jinxiao;Luo, Tingting;Tong, Li-Tao
关键词：Sour rice paste; Lactic fermentation; Organic acids; Volatiles; Bacterial community
-
A comprehensive review of starch-based technology for encapsulation of flavor: From methods, materials, and release mechanism to applications
作者：Shan, Yimeng;Li, Jiaxin;Nie, Mengzi;Li, Dezhi;Zhang, Yue;Li, Yang;Wang, Lili;Liu, Liya;Wang, Fengzhong;Tong, Li-Tao;Tong, Li-Tao
关键词：Encapsulation techniques; Flavor; Starch-based materials; Inclusion complex; Release mechanisms; Industry applications
-
Numerical Simulation Analysis and Research on Drag Reduction and Stability Enhancement Effect of Head Swing Based on Biomimetic Flexible Variants
作者：Qi, Debo;Li, Yang;Yang, Baisheng;Wu, Zhengyang;Li, Bo;Niu, Shichao
关键词：head swing; drag reduction; biomimetic flexible variants; radial drag; numerical simulation
-
The PAP Gene Family in Cotton: Impact of Genome-Wide Identification on Fiber Secondary Wall Synthesis
作者：Sun, Cong;Li, Weijie;Qi, Ruiqiang;Liu, Yangming;Wang, Xiaoyu;Gong, Juwu;Gong, Wankui;Pan, Jingtao;Li, Yang;Shi, Yuzhen;Yan, Haoliang;Shang, Haihong;Yuan, Youlu;Wang, Xiaoyu;Shang, Haihong
关键词：cotton; purple acid phosphatase (PAP); cotton fiber; expression analysis
-
Machine Learning-Driven Identification of Key Environmental Factors Influencing Fiber Yield and Quality Traits in Upland Cotton
作者：Souaibou, Mohamadou;Yan, Haoliang;Pan, Jingtao;Li, Yang;Shi, Yuzhen;Gong, Wankui;Shang, Haihong;Gong, Juwu;Yuan, Youlu;Yan, Haoliang;Pan, Jingtao;Li, Yang;Shi, Yuzhen;Shang, Haihong;Gong, Juwu;Yuan, Youlu;Dai, Panhong;Yuan, Youlu
关键词：cotton; machine learning; genotype environment interaction; SHAP interpretation; environmental factors
-
Coenzyme Q GhCoQ9 enhanced the salt resistance by preserving the homeostasis of mitochondrial in upland cotton
作者：Xu, Nan;Zhong, Yike;Chen, Zhuona;Zhang, Doudou;Han, Xu;Wei, Renhui;Wang, Panpan;Li, Xinru;Gong, Juwu;Li, Yang;Yan, Haoliang;Yuan, Youlu;Shang, Haihong;Xu, Nan;Zhong, Yike;Zhang, Yuanming;Yuan, Youlu;Shang, Haihong;Chen, Zhuona;Zhang, Doudou;Han, Xu;Wang, Panpan;Li, Xinru;Zhao, Yanpeng;Li, Gangling;Yang, Zheng;Yuan, Youlu;Shang, Haihong;Gao, Xu
关键词：Cotton; CoQ; GhCoQ9; Salt stress; Ultrastructure
