Toward the quantification of adeno-associated virus titer by electrochemical impedance spectroscopy
文献类型： 会议论文
第一作者： Junhyeong Wang
作者： Junhyeong Wang 1 ; Mahshid Hosseini 2 ; Shriarjun Shastry 3 ; Eduardo Barbieri 4 ; Wenning Chu 4 ; Stefano Menegatti 3 ; Michael A. Daniele 5 ;
作者机构： 1.Department of Electrical and Computer Engineering, NC State University, Raleigh, NC, USA
2.Joint Department of Biomedical Engineering, NC State University and UNC at Chapel Hill, Raleigh, NC, USA
3.Biomanufacturing Training and Education Center, NC State University, Raleigh, NC, USA|Department of Chemical & Biomolecular Engineering, NC State University, Raleigh, NC, USA
4.Department of Chemical & Biomolecular Engineering, NC State University, Raleigh, NC, USA
5.Department of Electrical and Computer Engineering, NC State University, Raleigh, NC, USA|Joint Department of Biomedical Engineering, NC State University and UNC at Chapel Hill, Raleigh, NC, USA
关键词： Electrodes;Surface impedance;Electric potential;Surface resistance;Dynamic range;Charge transfer;Biosensors
会议名称： 2023 IEEE BioSensors Conference
主办单位：
页码： 1-4
摘要： Gene therapies have shown great promise for the potential treatment of a broad range of diseases. Adeno-associated viruses (AAVs) are popular gene vectors because of their ability to target specific tissues, and they have demonstrated high transduction efficiencies in multiple neurological targets. While these therapeutics hold great promise, their biomanufacturing has limited potential cost-reduction and more widespread adoption. Herein, we report the preliminary development of an immunosensor for measuring the titer of adeno-associated virus 2 (AAV2), which may be deployed for rapid quantification of product yield during AAV biomanufacturing. We functionalized an interdigitated electrode array with anti-AAV2 antibodies, and electrochemical impedance spectroscopy was employed to investigate the response to AAV2 titer. A Faradaic sensing principle was utilized, in which the charge transfer resistance (Rct) of an electrochemical reporter was monitored after capture of AAV2 on the surface of the sensor. A linear response was measured over titers 1012 - 1013 capsids/mL.
分类号： q-53
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