文献类型： 会议论文

第一作者： Marina Y. Linova

作者： Marina Y. Linova ¹ ; Edite M. Tiago Gomes Martins ¹ ; Dan N. Pham ¹ ; Jinxin Fan ² ; Sobhana A. Sripada ² ; Stefano Menegatti ² ; Ruben G. Carbonell ³ ; John M. Woodley ¹ ;

作者机构： 1.Department of Chemical and Biochemical Engineering, Technical University of Denmark

2.Department of Chemical and Biomolecular Engineering, North Carolina State University

3.Department of Chemical and Biomolecular Engineering, North Carolina State University and Biomanufacturing Training and Education Center (BTEC), North Carolina State University

关键词： Protein recovery;Protein removal;Protein capture;Single-use devices;Process development;In-situ product removal

会议名称： Integrated Continuous Biomanufacturing Conference

主办单位：

页码： 62-62

摘要： Depending on how labile therapeutic proteins are they can be difficult to manufacture in traditional fed-batch processes due to their unstable nature, unfavorable upstream conditions, and degradation by host cell proteases. Integration of the protein capture step with the upstream process, in a continuous or semi-continuous operation, could offer a solution by rapidly recovering the target product or removing unwanted impurities in a flow-through mode. In this context, single-use devices for protein capture, such as membrane chromatography devices and functionalized chromatography resins, are attractive tools suitable for such processes. We investigate new strategies for the evaluation of novel single-use devices for their implementation in an integrated process, bridging the gap between the device development and process development.

分类号： q81-53