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Three-dimensional Ⅹ-ray microscopy of zebrafish larvae

文献类型： 会议论文

第一作者： Melissa Osterwalder

作者： Melissa Osterwalder 1 ; Jan Stephan Bolten 2 ; Griffin Rodgers 1 ; Georg Schulz 3 ; Christine Tanner 1 ; Emre Coerek 2 ; Joerg Huwyler 2 ; Bert Mueller 1 ;

作者机构： 1.Biomaterials Science Center, Department of Biomedical Engineering, University of Basel, Gewerbestrasse 14, 4123 Allschwil, Switzerland,Biomaterials Science Center, Department Clinical Research, c/o University Hospital Basel, Schanzenstrasse 55, 4031 Basel, Switzerland

2.Division of Pharmaceutical Technology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, 4056 Basel, Switzerland

3.Biomaterials Science Center, Department of Biomedical Engineering, University of Basel, Gewerbestrasse 14, 4123 Allschwil, Switzerland,Biomaterials Science Center, Department Clinical Research, c/o University Hospital Basel, Schanzenstrasse 55, 4031 Basel, Switzerland,Core Facility Micro- and Nanotomography, Department of Biomedical Engineering, University of Basel, Gewerbestrasse 14, 4123 Allschwil, Switzerland

关键词： Zebrafish embryo;hard Ⅹ-ray computed tomography;synchrotron radiation source;conventional Ⅹ-ray source;ethanol and paraffin embedding

会议名称： Conference on Bioinspiration, Biomimetics, and Bioreplication

主办单位：

页码： 115860J.1-115860J.13

摘要： Successful tomographic imaging of soft tissues with micrometer resolution includes preparation, acquisition, reconstruction, and data evaluation. Tissue preparation is essential for hard Ⅹ-ray microtomography, because staining- and embedding materials can substantially alter the biological tissue post mortem. We performed tomographic imaging of zebrafish embryos in alcohol and after paraffin embedding with a conventional Ⅹ-ray source and at a synchrotron radiation facility. The resulting multi-modal, three-dimensional data were registered for direct comparison. Single-cell precision was reached for the synchrotron radiation-based approach, which allows for segmentation of full organs such as the embryonic kidneys. While this approach offers an order of magnitude higher spatial resolution, many of the anatomical features can be readily recognized with the more accessible laboratory system. Propagation-based data acquisition enabled us to demonstrate the complementary nature of the edge-enhanced absorption contrast- and the phase contrast-based modality for visualizing multiple mi-croanatomical features. While ethanol and paraffin embeddings allowed for identification of the same anatomical structures, paraffin-embedding, however, led to more artefacts and shrinkage. The presented multi-modal imaging approaches can be further extended to visualize three to four orders of magnitude larger volumes such as adult zebrafish or complete organs of larger animals such as mouse brains. Going towards even larger volumes, such as the human brain, presents further challenges related to paraffin embedding, data acquisition and handling of the peta-byte scale data volumes. This study provided a multi-modal imaging strategy by the combination of Ⅹ-ray sources and sample embeddings which can play a role in addressing these challenges.

分类号： q81

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[2]Non-rigid registration to determine strain fields during mouse brain fixation and embedding. Bert Mueller,Christine Tanner,Griffin Rodgers,Georg Schulz,Willy Kuo,Mario Scheel,Vartan Kurtcuoglu,Timm Weitkamp. 2021

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