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Three-dimensional imaging of porcine joint tissues down to the subcellular level

文献类型： 会议论文

第一作者： Georg Schulz

作者： Georg Schulz 1 ; Andrea Barbero 2 ; Francine Wolf 2 ; Griffin Rodgers 3 ; Christine Tanner 3 ; Timm Weitkamp 4 ; Marcus Mumme 5 ; Marta Morawska 6 ; Daniel Beer 6 ; Bert Mueller 3 ;

作者机构： 1.Biomaterials Science Center, Department of Biomedical Engineering, University of Basel, Gewerbestrasse 14, 4123 Allschwil, Switzerland, Core Facility Micro- and Nanotomography, Department of Biomedical Engineering, University of Basel, Gewerbestrasse 14, 4123 Allschwil, Switzerland

2.Department of Biomedicine, Univeristy of Basel, Hebelstrasse 20, 4056 Basel, Switzerland

3.Biomaterials Science Center, Department of Biomedical Engineering, University of Basel, Gewerbestrasse 14, 4123 Allschwil, Switzerland

4.Synchrotron SOLEIL, L'Orme des Merisiers, 91190 Saint-Aubin, France

5.Regenerative Cartilage Surgery, University Hospital of Basel, Spitalstrasse 21, 4031 Basel

6.Advanced Osteotomy Tools, Wallstrasse 6, 4051 Basel, Switzerland

关键词： Porcine knee joint;calcified cartilage;chondrocytes;decalcification;ethanol fixation;paraffin embedding;synchrotron radiation;microtomography

会议名称： Conference on developments in X-ray tomography

主办单位：

页码： 1224215.1-1224215.10

摘要： Joint tissues consist of trabecular and cortical bone as well as calcified and hyaline cartilage, which presents a challenge for hard X-ray-based visualization on the sub-cellular level due to the wide range of local X-ray absorption values. The density of the calcified tissues requires rather high photon energy, which often leads to insufficient contrast within the cartilage and impedes the visualization of individual biological cells. Decalcification of the tissues reduces the total and local X-ray absorption values and allows for selecting a lower photon energy. Further contrast enhancement can be achieved by ethanol fixation and paraffin tissue embedding. In this study, we (ⅰ) searched for an appropriate visualization method to investigate lesions generated by a laser osteotome and (ⅱ) visualized a decalcified porcine joint after ethanol fixation and subsequent paraffin embedding using laboratory- and synchrotron radiation-based microtomography. The experiments at the ANATOMIX beamline of Synchrotron SOLEIL were performed in off-axis scan mode with a pixel size of 1.3 μm. Individual cells in all layers of the joint could be made visible and the effect of ethanol fixation and paraffin embedding demonstrated.

分类号： r312

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