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Non-destructive phase contrast hard X-ray imaging to reveal the three-dimensional microstructure of soft and hard tissues

文献类型： 会议论文

第一作者： Anna Khimchenko

作者： Anna Khimchenko 1 ; Georg Schulz 1 ; Hans Deyhle 1 ; Simone E. Hieber 1 ; Samiul Hasan 2 ; Christos Bikis 1 ; Joachim Schulz 2 ; Lo?c Costeur 3 ; Bert Müller 1 ;

作者机构： 1.University of Basel, Biomaterials Science Center, Department of Biomedical Engineering, Gewerbestrasse 14, 4123 Allschwil, Switzerland

2.Microworks GmbH, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany

3.Naturhistorisches Museum Basel, Augustinergasse 2, 4051 Basel, Switzerland

关键词： X-ray microtomography;phase contrast;grating interferometer set-up;laboratory-based microtomography systems

会议名称： SPIE Conference on Bioinspiration, Biomimetics, and Bioreplication

主办单位：

页码： 97970B-1-97970B-12

摘要： X-ray imaging in the absorption contrast mode is an established method of visualising calcified tissues such as bone and teeth. Physically soft tissues such as brain or muscle are often imaged using magnetic resonance imaging (MRI). However, the spatial resolution of MRI is insufficient for identifying individual biological cells within three-dimensional tissue. X-ray grating interferometry (XGI) has advantages for the investigation of soft tissues or the simultaneous three-dimensional visualisation of soft and hard tissues. Since laboratory microtomography (μCT) systems have better accessibility than tomography set-ups at synchrotron radiation facilities, a great deal of effort has been invested in optimising XGI set-ups for conventional μCT systems. In this conference proceeding, we present how a two-grating interferometer is incorporated into a commercially available nanotom? m (GE Sensing & Inspection Technologies GmbH) μCT system to extend its capabilities toward phase contrast. We intend to demonstrate superior contrast in spiders (Hogna radiata (Fam. Lycosidae) and Xysticus erraticus (Fam. Thomisidae)), as well as the simultaneous visualisation of hard and soft tissues. XGI is an imaging modality that provides quantitative data, and visualisation is an important part of biomimetics; consequently, hard X-ray imaging provides a sound basis for bioinspiration, bioreplication and biomimetics and allows for the quantitative comparison of biofabricated products with their natural counterparts.

分类号： N-532

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[1]X-ray grating interferometer for imaging at asecond-generation synchrotron radiation source. Julia Herzen,Felix Beckmann,Tilman Donath,Malte Ogurreck,Christian David,Franz Pfeiffer,Jurgen Mohr,Elena Reznikova,Stefan Riekehr,Astrid Haibel,GeorgSchulz,Bert Muller,Andreas Schreyer. 2010

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