文献类型： 会议论文

第一作者： Giuseppe Quero

作者： Giuseppe Quero ¹ ; Marco Consales ¹ ; Renato Severino ¹ ; Patrizio Vaiano ¹ ; Alessandra Boniello ¹ ; Annamaria Sandomenico ² ; Menotti Ruvo ² ; Anna Borriello ³ ; Laura Diodato ³ ; Simona Zuppolini ³ ; Michele Giordano ³ ; Immacolata Cristina Nettore ⁴ ; Annamaria Colao ⁴ ;

作者机构： 1.Optoelectronics Group, Dept. of Engineering, University of Sannio

2.Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale delle Ricerche (IBB-CNR) and Centro Interuniversitario di Ricerca sui Peptidi Bioattivi (CIRPeB)

3.Institute for Polymers, Composites and Biomaterials (IPCB)-CNR

4.Department of Clinical Medicine and Surgery, University of Napoli "Federico II"

关键词： Long Period Fiber Grating (LPG);Optical Fiber Biosensor;Reflection Type LPG;Thyroglobulin

会议名称： International Conference on Health Informatics

主办单位：

页码： 539-547

摘要： We report an innovative fiber optic biosensor based on Long Period Gratings (LPGs) working in reflection configuration for real time monitoring of human Thyroglobulin (Tg), a protein marker of differentiated thyroid cancer. A standard LPG is first transformed in a practical probe working in reflection mode, and then it is coated with a single layer of atactic polystyrene (aPS) in order to increase its surrounding refractive index sensitivity and to provide, at the same time, the desired interfacial properties for a stable anti-Tg antibody. The functionalized reflection-type LPG biosensor clearly demonstrates the effectiveness and sensitivity of the developed biosensing platform, allowing the real time and label-free detection of Tg in the needle washouts of fine-needle aspiration biopsies, at concentrations useful for pre- and post-operative assessment of the biomarker levels. Analyte recognition and capture were confirmed with a parallel on fiber ELISA-like assay using, in pilot tests, the biotinylated protein and HRP-labeled streptavidin for its detection. Dose-dependent experiments showed that the detection is linearly dependent on concentration within the range between 0 and 4 ng/mL, while antibody saturation occurs for higher protein levels.

分类号： G20-53