Real time analysis of the enzymatic digestion of chondroitin sulfate: role of the sulfation pattern
文献类型: 会议论文
第一作者: Carla Silva
作者: Carla Silva 1 ; Ramon Novoa-Carballal 1 ; Rui Reis 1 ; Iva Pashkuleva 1 ;
作者机构: 1.3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimaraes, Portugal
会议名称: European Conference on Biomaterials
主办单位:
页码: 387-
摘要: The catalytic profiles of the studied enzymes were determined: hyaluronidase is mainly endolytic whereas chondroitinase acts via both endolytic and exolytic mechanisms. Both enzymes showed preference for CS-C, however in a different extent. Medium sized polysaccharides are obtained with hyaluronidase whereas higher amount of low molecular species are obtained with chondroitinase. This protocol can be also applied for obtaining of well defined oligosaccharides in a large scale.
分类号: Q691
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