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Oxidative Stress Mediated Cytotoxicity of Perchlorate in Fish Primary Cultured Hepatocytes

文献类型: 会议论文

第一作者: Xiao-Hu Zhao

作者: Xiao-Hu Zhao 1 ; Pei-Jiang Zhou 1 ; Xiu Chen 1 ;

作者机构: 1.School of Resources and Environmental Sciences,Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory,Wuhan University,Wuhan 430079,China

关键词: Oxidative stress;Cytotoxicity;Perchlorate;Primary culture;Hepatocytes

会议名称: International conference on pollution ecology

主办单位:

页码: 26-27

摘要: Perchlorate (ClO4-) is a new threat to environment all through the world and occupational studies have manifested that it could inhibit thyroid hormone synthesis.To investigate whether the Perchlorate exposure causes cytotoxicity in fish by inducing oxidative stress,the primary cultured hepatocytes isolated from Carassius auratus were incubated with different concentrations (0-1000 mg L-1) of the Perchlorate at 25 ℃ for 12 h in vitro.The results demonstrated that apoptosis was involved in the cytotoxic effect of the Perchlorate and accompanied with generation of reactive oxygen species (ROS),Ca2+,Mg2+-ATPase activity decline,intracellular Ca2+ elevation and disruption of mitochondrial membrane potential (Δψm).

分类号: q958.116

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