文献类型: 会议论文
第一作者: Xu Jin
作者: Xu Jin 1 ; Lanxia Liu 1 ; Lin Mei 1 ; Xigang Leng 1 ; Chao Zhang 1 ; Hongfan Sun 1 ; Cunxian Song 1 ; R.J. Levy 2 ;
作者机构: 1.Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, The Tianjin Key Laboratory of Biomaterial Research, Tianjin 300192, People's Republic of China
2.The Children's Hospital of Philadelphia, Abramson Pediatric Research Center, Suite 702, 3516 Civic Center Boulevard, Philadelphia, PA 19104
关键词: Antibody;Collagen Matrix;Specific Gene Delivery
会议名称: Pacifichem 2005
主办单位:
页码: 243-264
摘要: We investigated the feasibility of immobilizing plasmid DNA on collagen matrix through covalently coupled anti-DNA antibody to achieve long-lasting and site-specific DNA delivery. Methods: Collagen was modified with SPDP (Nsuccinimidyl-3- (2-pyridyldithiol)-propionate) / DTT to introduce –SH group on collagen matrix. A pyridyl disulfide modified anti-DNA IgM wasthen covalently linked with collagen through thiol reaction. These antibodies enabled tethering of plasmid DNA through highly specific antigen-antibody affinity. Radioactive-labelled antibody and pDNA were used to evaluate binding capacity and stability. A plasmid EGFP-N3 was tethered on anti-DNA antibody-modified collagen-coated coronary stent as model gene delivery system that was assessed in both cell culture and in a rabbit model. Results showed that the amount of antibody bound on collagen films was 15 times higher than that of the control films (without SPDP chemical linking), and that the retention time of the antibody on films was also significantly longer. The pDNA-tethered and collagen-coated stents had no detrimental effect on cell growth. In cell culture studies, numerous GFP-transduced cells were detected only on the stent surface, thus demonstrating efficient and highly localized gene delivery. The overall GFP transduction efficiency in treated rabbit coronary arteries was 2.8±0.7% of the total cells. However, the rate of neointima transduction was about 7% of total cells in this region. Importantly, no distal spread of the vector was detectable by PCR, either in distal organs, or in the downstream segments of the stented arteries. In conclusion, we report the first successful use of anti-DNA antibody-modified stent as plasmid gene delivery system; this technique thus represents an efficient and highly localized gene delivery system both in vitro and in vivo.
分类号: R965
- 相关文献
[1]Development of Monoclonal Antibodies Recognizing Linear Epitope: Illustration by Three Bacillus thuringiensis Crystal Proteins of Genetically Modified Cotton, Maize, and Tobacco. Cao, Zhen,Zhang, Wei,Ning, Xiangxue,Wang, Baomin,Cao, Zhen,Li, Qing X.,Liu, Yunjun. 2017
[2]Humoral and cellular immune responses to Fasciola gigantica experimental infection in buffaloes. Zhang, WY,Moreau, E,Yang, BZ,Li, ZQ,Hope, JC,Howard, CJ,Huang, WY,Chauvin, A. 2006
[3]Developments on Immunoassays for Pyrethroid Chemicals. Jin, Maojun,Chen, Ge,Du, Pengfei,Zhang, Chan,Cui, Xueyan,She, Yongxin,Zheng, Lufei,Wang, Shanshan,Shao, Hua,Jin, Fen,Wang, Jing,Gee, Shirley J.,Hammock, Bruce D.,Gee, Shirley J.,Hammock, Bruce D.. 2017
[4]Effects of dietary vitamin E on immunological stress of layers and their offspring. Zhao, G. P.,Han, M. J.,Zheng, M. Q.,Zhao, J. P.,Chen, J. L.,Wen, J..
[5]Immunoassays for aflatoxins. Li, Peiwu,Zhang, Qi,Zhang, Wen.
[6]Experimental infection with bovine ephemeral fever virus and analysis of its antibody response cattle. Zheng, F. Y.,Chen, Q. W.,Li, Z.,Gong, X. W.,Wang, J. D.,Yin, H..
[7]Primary Babesia rodhaini infection followed by recovery confers protective immunity against B. rodhaini reinfection and Babesia microti challenge infection in mice. Wang, Guanbo,Efstratiou, Artemis,Moumouni, Paul Franck Adjou,Liu, Mingming,Jirapattharasate, Charoonluk,Guo, Huanping,Gao, Yang,Suzuki, Hiroshi,Igarashi, Ikuo,Xuan, Xuenan,Cao, Shinuo,Zhou, Mo.
[8]Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers. Xu, Lin,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Xu, Lin,Li, Peiwu,Xu, Lin,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Zhang, Qi,Li, Peiwu,Li, Peiwu. 2016
[9]Effects of Enterococcus faecium (SF68) on immune function in mice. Sun, Peng,Wang, Jiaqi,Jiang, Yanmei,Sun, Peng.
[10]Vaccination with Different M2e Epitope Densities Confers Partial Protection against H5N1 Influenza A Virus Challenge in Chickens. Liu, Ming.
[11]A sensitive electrochemical impedance immunosensor for determination of malachite green and leucomalachite green in the aqueous environment. Zhu, Dan,Pang, Xiumei,Liu, Yue,Wang, Xue,Chen, Gang,Li, Qiangqiang.
[12]Replacing antibodies with aptamers in lateral flow immunoassay. Chen, Ailiang,Yang, Shuming,Chen, Ailiang,Yang, Shuming.
[13]Protection of mice against homologous or heterologous infections with antiserum mixture to the predominant variable antigen type repertoire of Trypanosoma evansi YNB stock. Yang, Chunjiang,Suo, Xun,Huang, Xiaoxi,Zhang, Guangzhou,Jia, Yonggen,Wang, Quan,Shen, Jie. 2007
[14]Development of a sensitive ELISA for the analysis of the organophosphorous insecticide fenthion in fruit samples. Zhang, Qi,Sun, Qin,Hu, Baishi,Shen, Qing,Yang, Gang,Liang, Xiao,Sun, Xiao,Liu, Fengquan.
[15]A broad protection provided by matrix protein 2 (M2) of avian influenza virus. Meng, Qingwen,Liu, Guangliang,Liu, Yonggang,Wang, Wei,Chen, Hongyan,Deng, Xiaoxia,Xu, Kehui,Zheng, Xiangdong,Zhang, Dongwei,Pang, Hai,Liu, Guangliang.
[16]A simple and rapid immunochromatographic strip test for monitoring antibodies to H5 subtype Avian Influenza Virus. Cui, Shangjin,Chen, Changmu,Tong, Guangzhi. 2008
[17]Evaluating the immune responses of mice to subcutaneous immunization with Helicobacter pylori urease B subunit. Sun, Peng,Wang, Jia-Qi,Zhang, Yu-Tao,Zhao, Sheng-Guo.
[18]Development of a sensitive Luminex xMAP-based microsphere immunoassay for specific detection of Iris yellow spot virus. Yu, Cui,Yang, Cuiyun,Song, Shaoyi,Yu, Zixiang,Zhou, Xueping,Wu, Jianxiang,Zhou, Xueping. 2018
作者其他论文 更多>>
-
ReSel: N-ary Relation Extraction from Scientific Text and Tables by Learning to Retrieve and Select
作者:Jerry Junyang Cheung;Yue Yu;Yingjun Mou;Xiang Chen;Le Song;Chao Zhang;Yuchen Zhuang;Yinghao Li
关键词:
-
‘香野’草莓苗期枯萎症状病菌的分离及鉴定
作者:LEI Heng-shu;雷恒树;XU Jin;徐进
关键词:草莓苗期;枯萎症状;病菌分离;菌落鉴定
-
The Study of Animal Products Perceived Quality Based on Cue Theory - Data from Fu Jian Province
作者:Chao Zhang;Ganqiong Li;Xiaoxia Dong;Yumei Zhang;Zhemin Li
关键词:Consumer perceived quality;Animal products;Cue theory;Factor analysis;Differences in characteristics
-
SYNTHESIS AND PHYSICOCHEMICAL EVALUATION OF MALEIC ANHYDRIDE-GRAFTED-POLY(D,L-LACTIDE-CO-GLYCOLIDE) AS FUNCTIONAL STENT COATING FOR LOCALIZED GENE DELIVERY
作者:Guilei Ma;Mei Yu;Mingming Chen;Cunxian Song
关键词:localized gene delivery;covalent immobilization;PLGA;maleic anhydride;graft
-
RhGF-loaded Microspheres for the Treatment of Neurodegenerative Disorders
作者:Cunxian Song;Haigang Gu
关键词:
-
IDENTIFICATION OF EXOTIC GENETIC COMPONENTS AND DNA METHYLATION PATTERN ANALYSIS OF THREE COTTON INTROGRESSION LINES FROM Gossypium bickii
作者:Shou Pu He;Jun Ling Sun;Chao Zhang;Xiong Ming Du
关键词:Gossypium hirsutum;Gossypium bickii;introgression;AFLP;MSAP


