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The microstructure of mandibular bone graftsand three-dimensional cell clusters

文献类型： 会议论文

第一作者： Sarper Gurel

作者： Sarper Gurel 1 ; Corinne Unold 1 ; Hans Deyhle 1 ; Georg Schulz 1 ; Sebastian Kuhl 2 ; Belma Saldamli 3 ; Jutta Tubel 3 ; Rainer Burgkart 3 ; Felix Beckmann 4 ; Bert Muller 1 ;

作者机构： 1.Biomaterials Science Center, University of Basel, 4031 Basel, Switzerland

2.School of Dental Medicine, University of Basel, 4056 Basel, Switzerland

3.Department of Orthopedic Surgery, Klinikum r. d. Isar, TU Munchen, 81675 Munich, Germany

4.GKSS-Research Center, 21502 Geesthacht, Germany

关键词： three-dimensional cell cluster;bone graft;socket preservation;synchrotron radiation;micro computed tomography;component labeling;adipocyte

会议名称： SPIE Conference on Developments in X-ray Tomography

主办单位：

页码： 78041G:1-78041G:12

摘要： During the last decade, several tissues and biomaterials for medical applications in replacing bony tissues have been developed. Three-dimensional cell clusters and mandibular bone grafts are two distinct examples of these developments. The characterization of the complex three-dimensional structures, however, is still mainly restricted on the two-dimensional analysis of histological slices. The present paper examines the quantitative analysis of mandibular bone grafts and three-dimensional cell clusters on the basis of synchrotron radiation-based micro computed tomography measurements. An automated search of pre-defined microstructures through component labeling is applied to the real datasets in order to identify features that reside independently from other components. The examples demonstrate three levels of complexity: rather large pieces of bone augmentation material that touch each other, individual adipocytes difficult to automatically segment in a wet cluster and osmium-stained adipocyte exhibiting higher X-ray absorption than the surrounding tissue. Although the structures of interest such as the cells can be labeled, de-clustering of the components requires the incorporation of erosion and dilation algorithms.

分类号： N-532

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