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Enhancement of immune responses to classical swine fever virus E2 in mice by fusion or mixture with the porcine IL-28B

文献类型： 外文期刊

作者： Yuan, Hong 1 ; Jiao, Yunjuan 1 ; Gao, Jie 1 ; Wang, Tao 1 ; Xia, Yingju 2 ; Li, Kun 1 ; Yang, Yuxuan 1 ; Zhang, Jing 1 ; Bao, Huifang 1 ; Wang, Lihao 1 ; Sun, Pu 1 ; Li, Dong 1 ; Li, Pinghua 1 ; Cao, Yimei 1 ; Zhao, Zhixun 1 ; Liu, Zaixin 1 ; Lu, Zengjun 1 ; Liu, Yebing 2 ; Bai, Xingwen 1 ;

作者机构： 1.Lanzhou Univ, Lanzhou Vet Res Inst, Chinese Acad Agr Sci, Coll Vet Med, Lanzhou 730000, Peoples R China

2.China Inst Vet Drug Control, Natl Reference Lab Class Swine Fever, Beijing 100081, Peoples R China

3.Gansu Prov Res Ctr Basic Disciplines Pathogen Biol, Lanzhou 730046, Peoples R China

关键词： Classical swine fever virus; E2 subunit vaccine; IL-28B

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.3； 五年影响因子：5.1 ）

ISSN： 0175-7598

年卷期： 2025 年 109 卷 1 期

页码：

收录情况： SCI

摘要： The E2 subunit vaccine has been considered a promising alternative to an attenuated classical swine fever (CSF) vaccine. However, it fails to induce a good cellular immune response. Given that immunogenic adjuvants can regulate the cellular immunity to achieve a maximum efficacy against antigens, immunostimulatory effects of porcine IL-28B on the CSF virus (CSFV) E2 subunit vaccine were evaluated in the present study. We expressed recombinant proteins E2-IL28B, E2, and IL-28B using CHO-S mammalian cells as an antigen expression platform, and three types of CSFV E2 subunit vaccines based on antigens E2-IL28B, E2 + IL-28B, and E2 were prepared, respectively. We found that both E2-IL28B and E2 + IL-28B antigens exhibited superior immunogenicity with dramatically induced antibody titers and neutralizing antibody levels than the E2 alone. Moreover, E2-IL28B or E2 + IL-28B, instead of E2, boosted cellular immune responses via obviously increasing the percentages of CD3(+)CD4(+) T lymphocytes, promoting the lymphocyte proliferations, and enhancing the release of Th1-type cytokines. All results revealed that the inclusion of IL-28B, whether fused or mixed with E2, significantly elevated E2-induced immune potencies, suggesting that IL-28B could be used as a molecular adjuvant to optimize the design of E2 subunit vaccine for more effective controls of the CSF disease.

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