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Identification of a novel glycoside hydrolase family 8 xylanase from Deinococcus geothermalis and its application at low temperatures

文献类型： 外文期刊

作者： Wang, Tingting 1 ; Lin, Min 1 ; Yan, Yongliang 2 ; Jiang, Shijie 1 ; Dai, Qilin 1 ; Zhou, Zhengfu 2 ; Wang, Jin 1 ;

作者机构： 1.Southwest Univ Sci & Technol, Coll Life Sci & Engn, Mianyang 621000, Peoples R China

2.Chinese Acad Agr Sci, Biotechnol Res Inst, Natl Key Lab Agr Microbiol, Beijing 100081, Peoples R China

3.Chinese Acad Agr Sci, Biotechnol Res Inst, Key Lab Agr Microbiome MARA, Beijing 100081, Peoples R China

关键词： Cold-adapted enzyme; Deinococcus geothermalis; DgeoXyn; GH8; Xylanase

期刊名称：ARCHIVES OF MICROBIOLOGY （ 影响因子：2.3； 五年影响因子：2.6 ）

ISSN： 0302-8933

年卷期： 2024 年 206 卷 7 期

页码：

收录情况： SCI

摘要： Xylanase is the most important hydrolase in the xylan hydrolase system, the main function of which is beta-1,4-endo-xylanase, which randomly cleaves xylans to xylo-oligosaccharides and xylose. Xylanase has wide ranging of applications, but there remains little research on the cold-adapted enzymes required in some low-temperature industries. Glycoside hydrolase family 8 (GH8) xylanases have been reported to have cold-adapted enzyme activity. In this study, the xylanase gene dgeoxyn was excavated from Deinococcus geothermalis through sequence alignment. The recombinant xylanase DgeoXyn encodes 403 amino acids with a theoretical molecular weight of 45.39 kDa. Structural analysis showed that DgeoXyn has a (alpha/alpha)6-barrel fold structure typical of GH8 xylanase. At the same time, it has strict substrate specificity, is only active against xylan, and its hydrolysis products include xylobiose, xylotrinose, xytetranose, xylenanose, and a small amount of xylose. DgeoXyn is most active at 70 degree celsius and pH 6.0. It is very stable at 10, 20, and 30 degree celsius, retaining more than 80% of its maximum enzyme activity. The enzyme activity of DgeoXyn increased by 10% after the addition of Mn2+ and decreased by 80% after the addition of Cu2+. The Km and Vmax of dgeox were 42 mg/ml and 20,000 U/mg, respectively, at a temperature of 70 degree celsius and pH of 6.0 using 10 mg/ml beechwood xylan as the substrate. This research on DgeoXyn will provide a theoretical basis for the development and application of low-temperature xylanase.

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