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Gene editing, metabolomics, network pharmacology strategies to explore terpenoid content and anti-TMV activity in NtSPS1 knockout Nicotiana tabacum

文献类型： 外文期刊

作者： Dai, Jia-Meng 1 ; Zhang, Jian-Duo 1 ; Liu, Xin 1 ; Zhang, Ling-Fang 2 ; Wang, Jin 1 ; Xu, Yong 1 ; Yang, Guang-Yu 1 ; Li, Jing 1 ; Chen, Ming-Li 4 ; Hu, Qiu-Fen 1 ;

作者机构： 1.China Tobacco Yunnan Ind Co Ltd, Yunnan Key Lab Tobacco Chem, Kunming 650231, Peoples R China

2.Yunnan Minzu Univ, Key Lab Chem Ethn Med Resources, State Ethn Affairs Commiss, Kunming 650031, Peoples R China

3.Yunnan Minzu Univ, Minist Educ, Kunming 650031, Peoples R China

4.Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Peoples R China

关键词： Nicotiana tabacum; CRISPR/Cas9; NtSPS1; Terpenoid; Network pharmacology; anti-TMV activities

期刊名称：SCIENTIFIC REPORTS （ 影响因子：3.9； 五年影响因子：4.3 ）

ISSN： 2045-2322

年卷期： 2025 年 15 卷 1 期

页码：

收录情况： SCI

摘要： The content of terpenoids in tobacco can alter its resistance to TMV. NtSPS1, a pivotal structural gene in tobacco, is capable to regulate the terpenoid content. In this study, we investigated the effect of NtSPS1 knockout in HD on the content of terpenoids and the anti-TMV activity of this mutant using gene editing, widely targeted metabolomics, network pharmacology, and molecular docking. 48 terpenoids (six up-regulated and five down-regulated) in NtSPS1 knockout tobacco compared with WT leaves. Notably, solanesol was remarkable downregulation which was lowered by fourfold and compounds 1 (log2FC = 18.2), 8 (log2FC = 16.7) were significant upregulation between the mutants and wild-type line leaves. The 46 terpenoid's target network encompassed 150 nodes, 509 edges and their underlying mechanisms in the therapeutic management of TMV are discussed. Furthermore, the network pharmacology and molecular docking revealed that compounds 16, 18, 23, 27, and 36 exhibited significant affinity in their respective interactions. Ultimately, five compounds were assayed for their anti-TMV effects, noteworthily, compounds 36 showed potential anti-TMV activity. Above all, we adopted a multifaceted approach to gain a comprehensive understanding of the terpenoid content and anti-TMV properties in NtSPS1 knockout HD. It enlightens the therapeutic potential of NtSPS1 knockout tobacco and it is helpful to find undescribed anti-TMV activity inhibitors, as well as searching for new anti-TMV candidates from the mutants.

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