文献类型: 外文期刊
作者: Dai, Shutao 1 ; Hou, Jinna 1 ; Long, Yan 1 ; Wang, Jing 1 ; Li, Cong 1 ; Xiao, Qinqin 1 ; Jiang, Xiaoxue 1 ; Zou, Xiaoxia 1 ;
作者机构: 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hunan, Peoples R China
2.Henan Acad Agr Sci, Crop Designing Ctr, Zhenzhou 450002, Henan, Peoples R China
关键词: Brassicaceae;Brassica;Miniature inverted repeat transposable elements;Monkey King;Tourist-like MITE;DNA methylation;bna-miR6031
期刊名称:BMC PLANT BIOLOGY ( 影响因子:4.215; 五年影响因子:4.96 )
ISSN: 1471-2229
年卷期: 2015 年 15 卷
页码:
收录情况: SCI
摘要: Background: Miniature inverted repeat transposable elements (MITEs) are important components of eukaryotic genomes, with hundreds of families and many copies, which may play important roles in gene regulation and genome evolution. However, few studies have investigated the molecular mechanisms involved. In our previous study, a Tourist-like MITE, Monkey King, was identified from the promoter region of a flowering time gene, BnFLC.A10, in Brassica napus. Based on this MITE, the characteristics and potential roles on gene regulation of the MITE family were analyzed in Brassicaceae. Results: The characteristics of the Tourist-like MITE family Monkey King in Brassicaceae, including its distribution, copies and insertion sites in the genomes of major Brassicaceae species were analyzed in this study. Monkey King was actively amplified in Brassica after divergence from Arabidopsis, which was indicated by the prompt increase in copy number and by phylogenetic analysis. The genomic variations caused by Monkey King insertions, both intra-and inter-species in Brassica, were traced by PCR amplification. Genomic sequence analysis showed that most complete Monkey King elements are located in gene-rich regions, less than 3kb from genes, in both the B. rapa and A. thaliana genomes. Sixty-seven Brassica expressed sequence tags carrying Monkey King fragments were also identified from the NCBI database. Bisulfite sequencing identified specific DNA methylation of cytosine residues in the Monkey King sequence. A fragment containing putative TATA-box motifs in the MITE sequence could bind with nuclear protein(s) extracted from leaves of B. napus plants. A Monkey King-related microRNA, bna-miR6031, was identified in the microRNA database. In transgenic A. thaliana, when the Monkey King element was inserted upstream of 35S promoter, the promoter activity was weakened. Conclusion: Monkey King, a Brassicaceae Tourist-like MITE family, has amplified relatively recently and has induced intra-and inter-species genomic variations in Brassica. Monkey King elements are most abundant in the vicinity of genes and may have a substantial effect on genome-wide gene regulation in Brassicaceae. Monkey King insertions potentially regulate gene expression and genome evolution through epigenetic modification and new regulatory motif production.
- 相关文献
作者其他论文 更多>>
-
Genome-wide identification and expression analysis of the SAUR gene family in foxtail millet (Setaria italica L.)
作者:Ma, Xiaoqian;Dai, Shutao;Qin, Na;Zhu, Cancan;Li, Junxia;Qin, Jiafan
关键词:Setaria italic; SAUR gene family; Genome-wide identified; Expression profile; Abiotic stress
-
Single-cell RNA sequencing profiles reveal cell type-specific transcriptional regulation networks conditioning fungal invasion in maize roots
作者:Cao, Yanyong;Zhang, Xingrui;Sun, Suli;Dong, Zhenying;Zhu, Zhendong;Duan, Canxing;Cao, Yanyong;Ma, Juan;Han, Shengbo;Hou, Mengwei;Zhou, Xiaoxiao;Zhang, Lili;Cao, Yanyong;Wei, Xun;Dong, Zhenying;Long, Yan;Wan, Xiangyuan;Cao, Yanyong;Ku, Lixia;Tang, Jihua;Han, Shengbo;Ku, Lixia;Tang, Jihua;Zhang, Lili;Zhang, Zhanyuan J.;Li, Xiangdong;Zhang, Zhanyuan J.
关键词:maize stalk rot; scRNA-seq; Fusarium verticillioides; co-expression module; immune regulatory networks
-
Evasion strategies of porcine reproductive and respiratory syndrome virus
作者:Chen, Xin-xin;Qiao, Songlin;Li, Rui;Wang, Jing;Li, Xuewu;Zhang, Gaiping;Zhang, Gaiping
关键词:immune evasion; porcine reproductive and respiratory syndrome virus (PRRSV); innate immunity; adaptive immunity; miRNA
-
Promoter DNA hypermethylation of TaGli-gamma-2.1 positively regulates gluten strength in bread wheat
作者:Zhou, Zhengfu;Liu, Congcong;Qin, Maomao;Li, Wenxu;Hou, Jinna;Shi, Xia;Dai, Ziju;Lei, Zhensheng;Wu, Zhengqing;Liu, Congcong;Yao, Wen;Lei, Zhensheng;Li, Yang;Zhou, Zhengfu;Tian, Baoming;Lei, Zhensheng;Wu, Zhengqing
关键词:Wheat; Gluten strength; Gliadin; TaGli-gamma-2.1; DNA methylation
-
Notch signaling leads to a slower progression of embryonic myogenic differentiation in Landrace than in Langtang pigs
作者:Cai, Shufang;Hu, Bin;Luo, Chenglong;Cai, Shufang;Zhu, Qi;Duo, Tianqi;Wang, Xiaoyu;Tong, Xian;Luo, Xiaorong;Yuan, Renqiang;Chen, Yaosheng;Mo, Delin;Wang, Jing;Xing, Baosong
关键词:myogenic progenitor; MyoD; Pax7; embryonic myogenesis; differentiation
-
Uncovering the genetic basis of gluten aggregation parameters by genome-wide association analysis in wheat (Triticum aestivum L.) using GlutoPeak
作者:Wu, Zhengqing;Qiu, Hongxia;Tian, Zhaoran;Liu, Congcong;Qin, Maomao;Li, Wenxu;Yang, Pan;Tian, Baoming;Wei, Fang;Zhou, Zhengfu;Lei, Zhensheng;Hou, Jinna;Wu, Zhengqing;Qiu, Hongxia;Tian, Zhaoran;Tian, Baoming;Wei, Fang;Zhou, Zhengfu;Lei, Zhensheng;Hou, Jinna;Wen, Yao;Lei, Zhensheng;Zhou, Zhengfu;Lei, Zhensheng
关键词:Wheat; Gluten aggregation properties; GlutoPeak parameters; GWAS
-
Genome-wide association study reveals the genetic architecture for calcium accumulation in grains of hexaploid wheat (Triticum aestivum L.)
作者:Shi, Xia;Zhou, Zhengfu;Li, Wenxu;Qin, Maomao;Yang, Pan;Hou, Jinna;Lei, Zhensheng;Wu, Zhengqing;Lei, Zhensheng;Lei, Zhensheng;Wang, Jiansheng;Huang, Fangfang
关键词:Hexaploid wheat (Triticum aestivum L; ); Ca accumulation; superior alleles; pyramid breeding; genome-wide association analysis