文献类型： 外文期刊
作者： Xie, Long 1 ; Feng, Hu 1 ; Li, Zhifang 1 ; Li, Di 1 ; Yang, Xiali 1 ; Yuan, Tanglong 1 ; Yan, Nana 1 ; He, Chenfei 1 ; Zheng, Jitan 1 ; Zuo, Zhenrui 1 ; Zheng, Yaxuan 1 ; Cao, Yaqi 1 ; Lu, Yangqing 2 ; Xiong, Xing Yao 1 ; Zuo, Erwei 1 ;
作者机构： 1.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Minist Agr & Rural Affairs, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr,K, Shenzhen, Peoples R China
2.Guangxi Univ, Coll Anim Sci & Technol, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangxi Key Lab Anim Breeding Dis Control & Preven, Nanning 530004, Peoples R China
3.Huazhong Agr Univ, Coll Anim Sci & Technol, Wuhan, Peoples R China
期刊名称：GENOME BIOLOGY （ 影响因子：12.3； 五年影响因子：17.4 ）
ISSN： 1474-760X
年卷期： 2024 年 25 卷 1 期
页码：
收录情况： SCI
摘要： The FokI catalytic domain can be fused to various DNA binding architectures to improve the precision of genome editing tools. However, evaluation of off-target effects is essential for developing these tools. We use Genome-wide Off-target analysis by Two-cell embryo Injection (GOTI) to detect low-frequency off-target editing events in mouse embryos injected with FokI-based architectures. Specifically, we test FokI-heterodimers fused with TALENs, FokI homodimers fused with RYdCas9, or FokI catalytic domains alone resulting in no significant off-target effects. These FokI genome editing systems exhibit undetectable off-target effects in mouse embryos, supporting the further development of these systems for clinical applications.
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