文献类型： 外文期刊
作者： Wu, Li-yan 1 ; Siemens, Johannes 2 ; Li, Shi-kai 1 ; Ludwig-Mueller, Jutta 2 ; Gong, Ya-ju 1 ; Zhong, Li 1 ; He, Jiang 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Inst Hort, Yunnan 650205, Kunming, Peoples R China
2.Tech Univ Dresden, Dept Biol, D-01062 Dresden, Germany
关键词： Plasmodiophora brassicae;Brassicaceae;Clubroot pathogenesis;Differential gene expression;RT-PCR
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）
ISSN： 0304-4238
年卷期： 2012 年 133 卷
页码：
收录情况： SCI
摘要： Clubroot disease caused by Plasmodiophora brassicae is one of the most damaging diseases within the Brassicaceae family. To find landmarks for specific clubroot development stages, expression of several P. brassicae genes was analyzed during the infection process in a universally susceptible Brassica rapa line (cv. Granaat, ECD 05). The results revealed that the genes Pb-YPT, Pb-Brip9 and Pb-PSA were regulated during infection of P. Brassicae, Pb-Brip9 and Pb-PSA were expressed in early stages of infection. This study provides evidence that semi-quantitative RT-PCR could be used for discriminating developmental stages of P. brassicae in B. rapa. Stage-specific gene expression of P. brassicae should be studied in B. rapa roots due to longer disease development in comparison to Arabidopsis thaliana. (C) 2011 Elsevier B.V. All rights reserved.
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