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Purification and characterization of a protein with antifungal, antiproliferative, and HIV-1 reverse transcriptase inhibitory activities from small brown-eyed cowpea seeds

文献类型： 外文期刊

作者： Tian, Guo-Ting 1 ; Zhu, Meng Juan 2 ; Wu, Ying Ying 2 ; Liu, Qin 2 ; Wang, He Xiang 2 ; Ng, Tzi Bun 4 ;

作者机构： 1.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resource, Kunming, Peoples R China

2.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China

3.China Agr Univ, Dept Microbiol, Beijing 100094, Peoples R China

4.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China

关键词： antifungal protein;isolation;antiproliferative;HIV-1 reverse transcriptase;small brown-eyed cowpea seeds;Vigna unguiculata

期刊名称：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY （ 影响因子：2.431； 五年影响因子：2.124 ）

ISSN： 0885-4513

年卷期： 2013 年 60 卷 4 期

页码：

收录情况： SCI

摘要： A 36-kDa protein, with an N-terminal sequence highly homologous to polygalacturonase (PG) inhibiting proteins, was isolated from small brown-eyed cowpea seeds. The protein was unadsorbed on diethylaminoethyl cellulose but adsorbed on both Affi-gel blue gel and SP-sepharose. It inhibited mycelial growth in the fungus Mycosphaerella arachidicola with an half-maximal (50%) inhibitory concentration (IC50) of 3.3 mu M. It reduced [methyl-H-3] thymidine incorporation into MBL2 lymphoma and L1210 leukemia cells with an IC50 of 7.4 and 5.4 mu M, respectively. It inhibited human immunodeficiency virus type 1 (HIV-1) reverse transcriptase with an IC50 of 12.9 mu M. However, it did not inhibit PG. The potent antifungal and antitumor activities of the protein suggest that it can be developed into an antifungal agent for combating M. arachidicola invasion in crops and an agent for cancer therapy in humans.

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