A molecular switch OsWRKY10-OsVQ8 orchestrates rice diterpenoid phytoalexin biosynthesis for broad-spectrum disease resistance
文献类型： 外文期刊
作者： Lin, Xianhui 1 ; Ding, Chaohui 1 ; Xiao, Wei 1 ; Wang, Jinhao 1 ; Lin, Zhuo 1 ; Sun, Xinli 1 ; Li, Suhua 2 ; Pan, Zhiqiang 4 ; Zeng, Rensen 1 ; Song, Yuanyuan 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Coll Agr, State Key Lab Agr & Forestry Biosecur, Key Lab,Minist Educ Genet,Breeding & Multiple Util, Fuzhou 350002, Peoples R China
2.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Guangdong Lab Lingnan Modern Agr, Shenzhen Branch,Key Lab Synthet Biol,Minist Agr &, Shenzhen 518000, Peoples R China
3.Fujian Agr & Forestry Univ, Key Lab Crop Biotechnol Fujian Higher Educ Inst, Key Lab Biol Breeding Fujian & Taiwan Crops, Minist Agr & Rural Affairs, Fuzhou 350002, Peoples R China
4.ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA
关键词：
disease resistance; diterpenoid phytoalexins;
期刊名称：NEW PHYTOLOGIST （ 影响因子：8.1； 五年影响因子：10.3 ）
ISSN： 0028-646X
年卷期： 2025 年 246 卷 5 期
页码：
收录情况： SCI
摘要： Rice plants synthesize a unique group of diterpenoid phytoalexins (DPs) that exhibit broad-spectrum antimicrobial activities and are biosynthesized by enzymes encoded by three biosynthetic gene clusters. However, the regulatory mechanisms of their biosynthesis remain unclear. Here, the regulatory roles of the transcription factor OsWRKY10 and its interacting VQ motif-containing protein OsVQ8 in DPs biosynthesis and disease resistance were investigated via genetic and biochemical analyses. Their CRISPR/Cas9-mediated knockout and over-expressing (OE) lines, as well as crossed lines WRKY10OE/vq8, were generated. OsVQ8 phosphorylation by mitogen-activated protein kinase (MAPK) cascades was examined. We found that OsWRKY10 co-expresses with and activates a specific set of genes involved in DPs biosynthesis, thereby enhancing DPs accumulation and disease resistance against both fungal blast and bacterial blight. We demonstrate that OsWRKY10 interacts with the VQ motif-containing protein OsVQ8, modulating DPs biosynthesis through OsVQ8 phosphorylation by the activated OsMKK4-OsMPK6 cascade upon perception of pathogen-associated molecular patterns. Our findings highlight how the interaction between OsVQ8 and OsWRKY10 serves as a molecular switch to regulate gene clusters and the entire pathway of DPs biosynthesis in rice and provides valuable insights for genetic engineering aimed at enhancing phytoalexin production and broad-spectrum disease resistance in staple food crops.
- 相关文献
作者其他论文 更多>>
-
Strigolactone and Karrikin Signaling Influence the Recruitment of Wild Tobacco's Root Microbiome in the Desert
作者：Cheng, Jie;Luo, Shuai;Cheng, Xu;Li, Suhua;Cheng, Jie;Li, Suhua;Baldwin, Gundega;Baldwin, Ian T.
关键词：desert tobacco; root-associated microbiome; field-work; strigolactones; karrikins
-
Presence of Multiple Genetic Mutations Related to Insecticide Resistance in Chinese Field Samples of Two Phthorimaea Pest Species
作者：Zhu, Jiang;Liu, Juan;Lin, Weichao;Liang, Jiaxin;Li, Suhua;Zhu, Jiang;Chen, Ruipeng;Gao, Yulin;Nauen, Ralf
关键词：Phthorimaea operculella; Phthorimaea absoluta; target resistance; Ace1; VGSC; RDL
-
Root colonization by beneficial rhizobacteria
作者：Liu, Yunpeng;Shu, Xia;Zhang, Ruifu;Xu, Zhihui;Xun, Weibing;Chen, Yu;Sun, Xinli;Wang, Zhengqi;Ren, Yi;Shen, Qirong;Zhang, Ruifu;Chen, Lin;Shu, Xia
关键词：rhizosphere; bacteria; root colonization; plant-microbe interactions; root exudates
-
Mercaptoimidazole-capped gold nanoparticles as a potent agent against plant pathogenic fungi
作者：Xu, Tang;Wang, Cuixia;Li, Chong;Xu, Tang;Du, Ran;Li, Suhua;Yan, Jianbin;Hao, Wenshuai;Cha, Ruitao;Xu, Tang;Dai, Dai;Lin, Carol Sze Ki;Hao, Wenshuai;Cha, Ruitao
关键词：
-
Diel, seasonal, and inter-annual variation in carbon dioxide effluxes from lakes and reservoirs
作者：Golub, Malgorzata;Koupaei-Abyazani, Nikaan;Desai, Ankur R.;Vesala, Timo;Mammarella, Ivan;Lohila, Annalea;Vesala, Timo;Ojala, Anne;Bohrer, Gil;Weyhenmeyer, Gesa A.;Blanken, Peter D.;Eugster, Werner;Koebsch, Franziska;Sachs, Torsten;Chen, Jiquan;Czajkowski, Kevin;Deshmukh, Chandrashekhar;Guerin, Frederic;Heiskanen, Jouni;Humphreys, Elyn;Jonsson, Anders;Karlsson, Jan;Kling, George;Lee, Xuhui;Liu, Heping;Lohila, Annalea;Lundin, Erik;Morin, Tim;Podgrajsek, Eva;Provenzale, Maria;Sahlee, Erik;Serca, Dominique;Shao, Changliang;Spence, Christopher;Strachan, Ian B.;Xiao, Wei
关键词：eddy covariance; freshwater systems; lakes; reservoirs; carbon flux; synthesis
-
Adaptive evolution of the enigmatic Takakia now facing climate change in Tibet
作者：Hu, Ruoyang;Li, Xuedong;Hu, Yong;Zhang, Runjie;Lv, Qiang;Zhang, Min;Sheng, Xianyong;Zhao, Feng;Chen, Zhijia;Ding, Yuhan;Yuan, Huan;Wu, Xiaofeng;Xing, Shuang;Yan, Xiaoyu;Bao, Fang;Wan, Ping;Xiao, Lihong;Wang, Xiaoqin;Xiao, Wei;He, Yikun;Decker, Eva L.;van Gessel, Nico;Renault, Hugues;Wiedemann, Gertrud;Horst, Nelly A.;Reski, Ralf;Haas, Fabian B.;Wilhelmsson, Per K. I.;Ullrich, Kristian K.;Neumann, Eva;Rensing, Stefan A.;Lv, Bin;Liang, Chengzhi;Du, Huilong;Lu, Hongwei;Gao, Qiang;Cheng, Zhukua;You, Hanli;Xin, Peiyong;Chu, Jinfang;Liang, Chengzhi;Liang, Chengzhi;Liu, Yang;Gao, Qiang;Zheng, Liangsheng;Chen, Fei;Duan, Fuzhou;Zhao, Wenji;Li, Kai;Li, Zhongfeng;Li, Xingru;Cui, Hengjian;Zhang, Yong E.;Hu, Ruoyang;Ma, Chuan;Zhu, Ruiliang;Jia, Yu;Wang, Meizhi;Hasebe, Mitsuyasu;Ma, Hong;Xiao, Lihong;Renault, Hugues;Wiedemann, Gertrud;Horst, Nelly A.;Lv, Bin;Du, Huilong;Lu, Hongwei;Cheng, Zhukua;You, Hanli;Liu, Yang;Dong, Shanshan;Liu, Yang;Dong, Shanshan;Liu, Yang;Rensing, Stefan A.;Reski, Ralf;Ullrich, Kristian K.;Liang, Chengzhi;Chu, Jinfang;Huang, Chien-Hsun;Hasebe, Mitsuyasu;Hu, Ruoyang;Ullrich, Kristian K.;Liang, Chengzhi;Chu, Jinfang
关键词：
-
Field-work reveals a novel function for MAX2 in a native tobacco's high-light adaptions
作者：Li, Suhua;Lu, Ruirui;Meng, Shuaishuai;Li, Suhua;Baldwin, Gundega;Yang, Caiqiong;Wang, Ming;Baldwin, Ian T.;Huang, Jianbei;Wang, Ming
关键词：field-based phenotyping; Nicotiana attenuata
