Identification of neutralizing antibodies against monkeypox virus using high-throughput sequencing of A35+H3L+B cells from patients with convalescent monkeypox
文献类型： 外文期刊
作者： Hou, Ruitian 1 ; Jiang, Qiwei 2 ; Cheng, Meiling 3 ; Dai, Jun 1 ; Yang, Huiqin 1 ; Yuan, Jiao 4 ; Li, Xiao 2 ; Tang, Xiaoping 1 ; Yu, Haisheng 1 ;
作者机构： 1.Guangzhou Med Univ, Guangzhou Peoples Hosp 8, Inst Infect Dis, Guangzhou 510440, Peoples R China
2.Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun 130122, Peoples R China
3.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Ctr Artificial Intelligence Biol, Dept Bioinformat & Syst Biol,Key Lab Mol Biophys,M, Wuhan 430074, Peoples R China
4.Guangzhou Natl Lab, 9 XingDaoHuanBei Rd,Guangzhou Int Bio Isl, Guangzhou 510005, Guangdong, Peoples R China
5.Guangzhou Med Univ, GMU GIBH Joint Sch Life Sci, Guangdong Hong Kong Macau Joint Lab Cell Fate Regu, Hong Kong 511436, Peoples R China
关键词： Monkeypox virus; potent neutralizing antibody; A35 protein
期刊名称：VIRUS RESEARCH （ 影响因子：2.5； 五年影响因子：3.2 ）
ISSN： 0168-1702
年卷期： 2024 年 347 卷
页码：
收录情况： SCI
摘要： The global monkeypox virus (MPXV) outbreak in 2022 emphasizes the urgent need for effective and accessible new-generation vaccines and neutralizing antibodies. Herein, we identified MPXV-neutralizing antibodies using high-throughput single-cell RNA and V(D)J sequencing of antigen-sorted B cells from patients with convalescent monkeypox. IgG1-expressing B cells were obtained from 34 paired heavy- and light-chain B cell receptor sequences. Subsequently, three potent neutralizing antibodies, MV127, MV128, and MV129, were identified and reacted with the MPXV A35 protein. Among these, MV129, which has a half-maximal inhibitory concentration of 2.68 mu g/mL against authentic MPXV, was considered to be the putative candidates for MPXV neutralization in response to monkeypox disease.
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