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Transcriptomic analysis reveals the impact of Rutin on the proliferation, barrier function, and transcription of Porcine IPEC-J2

文献类型： 外文期刊

作者： Wang, Binbin 1 ; Huo, Lele 1 ; Tian, Wanru 1 ; Xing, Wenwen 1 ; Luo, Gang 1 ; Li, Mengxuan 1 ; Sun, Liumei 1 ; Liu, Jiying 1 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural & Anim Biotechnol, Zhenjiang 212100, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Sci Res Ctr, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr & Rural Affairs, Zhenjiang 212100, Peoples R China

关键词： Rutin; IPEC-J2 cells; Gut health; Transcriptome sequencing

期刊名称：VETERINARY RESEARCH COMMUNICATIONS （ 影响因子：2.0； 五年影响因子：2.1 ）

ISSN： 0165-7380

年卷期： 2025 年 49 卷 5 期

页码：

收录情况： SCI

摘要： The gastrointestinal health of livestock is essential for the agricultural sector. Rutin, a natural flavonoid extract from plants, exhibits antioxidant and anti-inflammatory properties, alongside a protective impact on the intestinal tract of pigs. However its influence on the proliferation, migration, and cellular junctions of IPEC-J2 cells remains unexplored. This study involved treating IPEC-J2 cells with varying concentrations of rutin. It was observed that after 24 h of treatment with 200 mu M rutin, cell viability was significantly increased, along with significant upregulation in the mRNA expression levels of proliferation-related genes (CCNE2, CDK4, CDK2, CCNDB, and PCNA) and tight junction genes (ZO-1, Occludin, and Claudin-1). Additionally, the expression of proliferation-related PCNA protein was significantly increased. Transcriptome sequencing revealed that rutin treatment impacted the overall transcription profile of IPEC-J2 cells, altering the expression of genes involved in critical physiological processes such as redox balance, cell proliferation, and tight junctions maintenance. Consequently, rutin demonstrated a significant enhancement in the proliferation of IPEC-J2 cells and improved the intestinal barrier function in pigs, offering valuable insights for sustaining pig intestinal health in swine husbandry.

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