Synthetic, marine, light-driven, autotroph-heterotroph co-culture system for sustainable (3-caryophyllene production
文献类型： 外文期刊
作者： Chen, Wenchao 1 ; Park, Young-Kyoung 2 ; Studena, Lucie 2 ; Bell, David 5 ; Hapeta, Piotr 2 ; Fu, Jing 2 ; Nixon, Peter J. 6 ; Ledesma-Amaro, Rodrigo 2 ;
作者机构： 1.Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Peoples R China
2.Imperial Coll London, Bezos Ctr Sustainable Prot, Dept Bioengn, Microbial Food Hub, London SW72AZ, England
3.Imperial Coll London, Ctr Synthet Biol, London SW72AZ, England
4.Univ Paris Saclay, Micalis Inst, INRAE, AgroParisTech, F-78350 Jouy En Josas, France
5.Imperial Coll London, SynbiCITE Innovat & Knowledge Ctr, London SW7 2AZ, England
6.Imperial Coll London, Dept Life Sci, London SW7 2AZ, England
关键词： Artificial light-driven consortia; Marine cyanobacteria; Yarrowia lipolytica; Sucrose; (3-Caryophyllene; Metabolic engineering; Synthetic biology; Engineering biology; Bioproduction; Precision fermentation
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.7； 五年影响因子：9.4 ）
ISSN： 0960-8524
年卷期： 2024 年 410 卷
页码：
收录情况： SCI
摘要： Applying low-cost substrate is critical for sustainable bioproduction. Co-culture of phototrophic and heterotrophic microorganisms can be a promising solution as they can use CO2 and light as feedstock. This study aimed to create a light-driven consortium using a marine cyanobacterium Synechococcus sp. PCC 7002 and an industrial yeast Yarrowia lipolytica. First, the cyanobacterium was engineered to accumulate and secrete sucrose by regulating the expression of genes involved in sucrose biosynthesis and transport, resulting in 4.0 g/L of sucrose secretion. Then, Yarrowia lipolytica was engineered to efficiently use sucrose and produce (3-caryophyllene that has various industrial applications. Then, co- and sequential-culture were optimized with different induction conditions and media compositions. A maximum (3-caryophyllene yield of 14.1 mg/L was obtained from the co-culture. This study successfully established an artificial light-driven consortium based on a marine cyanobacterium and Y. lipolytica, and provides a foundation for sustainable bioproduction from CO2 and light through co-culture systems.
- 相关文献
作者其他论文 更多>>
-
Unraveling the microecological mechanisms of phosphate-solubilizing Pseudomonas asiatica JP233 through metagenomics: insights into the roles of rhizosphere microbiota and predatory bacteria
作者：Tang, Yuhan;Wang, Linlin;Fu, Jing;Zhou, Fangyuan;Wu, Xiaoqing;Fan, Susu;Zhang, Xinjian;Wei, Hailei
关键词：phosphate-solubilizing bacteria;
Pseudomonas asiatica ; soil P cycling; metagenomics; predatory bacteria -
An extended processing strategy for rapeseed: Concentrating homologous phenolics from meal to enhance oil quality
作者：Liu, Pei;Liang, Duweina;Chen, Wenchao;Ni, Wei;Fu, Jing;Fu, Jing;Sun, Manman;Ding, Xiaoxia
关键词：Antioxidation mechanism; Homologous phenolics; Phenolic-abundant rapeseed oil; Post-process or extensional strategy; Rapeseed meal
-
Hyper-production of porcine contagious pleuropneumonia subunit vaccine proteins in Escherichia coli by developing a bicistronic T7 expression system
作者：Sun, Manman;Li, An;Bai, Zhonghu;Liu, Xiuxia;Sun, Manman;Ledesma-Amaro, Rodrigo;Sun, Manman;Ledesma-Amaro, Rodrigo;Sun, Manman;Wang, Peng;Gao, Alex Xiong;Chen, Wenchao
关键词：bicistronic T7 expression system; Escherichia coli; hyper-production; PCP subunit vaccine protein; recombinant expression
-
Stem lodging Resistance-1 controls stem strength by positively regulating the biosynthesis of cell wall components in Capsicum annuum L.
作者：Li, Qing;Fu, Canfang;Yang, Bozhi;Yu, Huiyang;He, Huan;Xu, Qing;Zou, Xuexiao;Hu, Bowen;Ou, Lijun;Li, Qing;Fu, Canfang;Yang, Bozhi;Yu, Huiyang;He, Huan;Xu, Qing;Zou, Xuexiao;Hu, Bowen;Ou, Lijun;Li, Qing;Miao, Wu;Liu, Rongyun;Chen, Wenchao;Zhang, Zhuqing
关键词：
-
Beneficial Effects of Epigallocatechin Gallate in Preventing Skin Photoaging: A Review
作者：Sun, Jiaqiang;Fu, Jing;He, Linlin;Guo, Xinmiao;Ye, Hua;Yin, Cuiyuan;Sun, Jiaqiang;Fu, Jing;He, Linlin;Sun, Jiaqiang;Fu, Jing;He, Linlin;Jiang, Yuelu;Li, Hongbo;Fu, Jing;Jiang, Heyuan
关键词：ultraviolet (UV); skin photoaging; epigallocatechin gallate (EGCG); beneficial effects
-
Algal oil alleviates antibiotic-induced intestinal inflammation by regulating gut microbiota and repairing intestinal barrier
作者：Zhang, Huimin;Xu, Zhenxia;Chen, Wenchao;Huang, Fenghong;Yang, Chen;Zhang, Huimin;Chen, Shouwen;Huang, Fenghong;Yang, Chen;Wang, Xu
关键词：algal oil; polyunsaturated fatty acids; intestinal barrier; intestinal inflammation; gut microbiota
-
Biorefining of rapeseed meal: A new and sustainable strategy for improving Cr(VI) biosorption on residual wastes from agricultural byproducts after phenolic extraction
作者：Liu, Pei;Gong, Yangmin;Yang, Chen;Deng, Shiyu;Chen, Wenchao;Liu, Pei;Gong, Yangmin;Yang, Chen;Deng, Shiyu;Chen, Wenchao;Ledesma-Amaro, Rodrigo;Park, Young-Kyoung;Ledesma-Amaro, Rodrigo;Park, Young-Kyoung;Wang, Yuliang;Wei, Hongbo
关键词：Rapeseed byproducts; Phenolic extract; Residual waste; Cr(VI) biosorption; Conjugational or free form of phenolics
