您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genome wide identification of Dof transcription factors in Carmine radish reveals RsDof33 role in cadmium stress and anthocyanin biosynthesis

文献类型： 外文期刊

作者： Gou, Caiming 1 ; Li, Jia 1 ; Chen, Bo 1 ; Cheng, Guoting 2 ; Zheng, Zhangfei 3 ; Peng, Hua 5 ; El-Sappah, Ahmed H. 1 ;

作者机构： 1.Yibin Univ, Sch Agr Forestry & Food Engn, Yibin 644000, Sichuan, Peoples R China

2.Yanan Univ, Coll Life Sci, Shaanxi Key Lab Res & Utilizat Resource Plants Loe, Yanan 716000, Shaanxi, Peoples R China

3.Fujian Agr & Forestry Univ, Coll Agr, Fuzhou 350000, Fujian, Peoples R China

4.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China

5.Sichuan Tourism Coll, Res Ctr Tourism Agr Dev, Chengdu 610100, Sichuan, Peoples R China

6.Zagazig Univ, Fac Agr, Genet Dept, Zagazig 44511, Egypt

关键词： Raphanus sativus L.; Dof transcription factors; Anthocyanin biosynthesis; Expression profile: OERsDof33

期刊名称：SCIENTIFIC REPORTS （ 影响因子：3.9； 五年影响因子：4.3 ）

ISSN： 2045-2322

年卷期： 2025 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Carmine radish (Raphanus sativus L.) is cultivated in Fuling, Chongqing, for its red color. Dof-TFs are critical in regulating plant growth, development, stress responses, and signal transduction.This work comprehensively examined the structure, evolution, and expression of the carmine radish Dof gene and its behavior under cadmium (Cd) stress. The radish genome has 59 RsDofs, which are divided into nine clusters (A: 8, B1: 10, B2: 10, C1: 3, C2.1: 5, C2.2: 4, C3: 11, D1: 4, and D2: 4). Phylogenetic tree analysis revealed significant Dof gene family resemblance between Arabidopsis thaliana and Brassica napus. Perhaps segment duplication resulted in RsDof gene family expansion. Cd stress-induced RsDof expression patterns were studied using an RNA-seq atlas and qRT-PCR. The majority of RsDofs were tissue-specific and Cd-sensitive. The involvement of RsDof genes in Cd stress response and anthocyanin synthesis was verified using qRT-PCR. RsDof33 is involved in Cd stress response and anthocyanin synthesis. A. thaliana overexpressed the recombinant fusion protein RsDof33-GFP, which was localized to the nucleus, resulting in fewer rosette leaves, delayed flowering, and higher anthocyanin concentration. RsDof33-expressing plants had significantly higher transcript levels of the auxin biosynthetic genes YUCCA (AtYUC2), auxin efflux carrier (AtPIN4), and AtKNAT2, which are involved in leaf shape development, as well as AtPAL, AtCHS, AtCHI, AtDFR, AtLDOX, and AtUF3GT. These findings indicate that RsDofs are critical to plant development and stress responses.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台