您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A deletion variant within the FGF5 gene in goats is associated with gene expression levels and cashmere growth

文献类型： 外文期刊

作者： Li, Yefang 1 ; Song, Shen 1 ; Zhang, Zhengkai 1 ; Liu, Xuexue 1 ; Zhang, Yanli 1 ; E, Guangxin 6 ; Ma, Yuehui 1 ; Jiang, Lin 1 ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Inst Anim Sci, Minist Agr, Lab Anim Poultry Genet Breeding & Reprod, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci CAAS, Inst Anim Sci, CAAS ILRI Joint Lab Livestock & Forage Genet Reso, Beijing, Peoples R China

3.Chinese Acad Med Sci & Peking Union Med Coll, Natl Ctr Cardiovasc Dis, Fuwai Hosp, State Key Lab Cardiovasc Dis, Beijing, Peoples R China

4.Yunnan Agr Univ, Fac Anim Sci & Technol, Kunming, Yunnan, Peoples R China

5.Univ Paul Sabatier, Ctr Anthropobiol & Genom Toulouse, Toulouse, France

6.Southwest Univ, Chongqing Engn Res Ctr Herbivores Resource Protec, Chongqing Key Lab Forage & Herbivore, Coll Anim Sci & Technol, Chongqing, Peoples R China

关键词： cashmere length; cis-acting element; deletion variant; FGF5; goat

期刊名称：ANIMAL GENETICS （ 影响因子：2.884； 五年影响因子：2.92 ）

ISSN： 0268-9146

年卷期：

页码：

收录情况： SCI

摘要： The FGF5 gene has been associated with the regulation of fibre length in mammals, including cashmere goats. A deletion variant at similar to 14kb downstream of the FGF5 gene showed significant divergence between cashmere and noncashmere goats in previous studies. In this study, we designed specific primers to genotype the deletion variant. The results of gel electrophoresis and Sanger sequencing revealed that a 507-bp deletion mutation is located at 95 454685-95455 191 of chromosome 6 in goats. Genotyping data from a large panel of 288 goats showed that the deletion at the FGF5 gene locus appeared to be associated with cashmere length. The deletion variant was close to fixation (frequency 0.97) in cashmere goats. Furthermore, electrophoretic mobility shift assays for evaluating DNA-protein interaction and mRNA expression levels of FGF5 suggested that the deletion variant may serve as a cis-acting element by specifically binding transcription factors to mediate quantitative changes in FGF5 mRNA expression. Our study illustrates how a structural mutation of the FGF5 gene has contributed to the cashmere growth phenotype in domestic goats. The deletion mutation within the FGF5 gene could potentially serve as a molecular marker of cashmere growth in cashmere goat breeding.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台