文献类型： 外文期刊
作者： Xu, Mengmeng 1 ; Huang, Mengmeng 1 ; Wang, Guodong 1 ; Han, Jingzhe 1 ; Yu, Hangbo 1 ; Zhang, Yulong 1 ; Liu, Runhang 1 ; Wu, Ziwen 1 ; Cui, Hongyu 1 ; Zhang, Yanping 1 ; Wang, Suyan 1 ; Liu, Yongzhen 1 ; Chen, Yuntong 1 ; Duan, Yulu 1 ; Li, Liuan 3 ; Gao, Yulong 1 ; Qi, Xiaole 1 ;
作者机构： 1.Chinese Acad Agr Sci, Avian Immunosuppress Dis Div, State Key Lab Anim Dis Control & Prevent, Harbin Vet Res Inst, Harbin 150069, Peoples R China
2.Tianjin Agr Univ, Coll Anim Sci & Vet Med, Tianjin Key Lab Agr Anim Breeding & Hlth Husb, Tianjin 300392, Peoples R China
3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China
4.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Harbin 150069, Peoples R China
关键词： Chicken NLRP3; Monoclonal antibody; Antigenic epitopes; Inflammation
期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）
ISSN： 0032-5791
年卷期： 2025 年 104 卷 11 期
页码：
收录情况： SCI
摘要： Inflammation is key important pathogenic response that occurs in several poultry diseases. NOD-like receptor protein 3 inflammasome plays a central role in initiating inflammation. However, the lack of antibodies against chicken-derived inflammatory factors presents a major bottleneck in related studies. In this study, we developed a monoclonal antibody (mAb) against chicken NLRP3 using hybridoma technology. This antibody can recognize both eukaryotic and prokaryotic NLRP3 proteins as well as the upregulation of endogenous NLRP3 in HD11 cells induced by lipopolysaccharide or infectious bursal disease virus. Furthermore, a novel antigenic epitope, 42DELEKVTHPSS52, located in the PYD domain of chicken NLRP3 and specifically recognized by this mAb was identified. This epitope is unique to chickens and valuable for distinguishing chicken NLRP3 from orthologs in other species. The developed mAb provides an important tool for detecting chicken NLRP3 and facilitates further study of its antigenic structure and biochemical characteristics.
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