Identification and characterization of aldo-keto reductase responsible for patulin degradation in Saccharomyces cerevisiae
文献类型： 外文期刊
作者： Yang, Chao 1 ; Huang, Lingxuan 1 ; Hu, Chen 1 ; Yao, Jieqiong 1 ; Zhou, Ting 2 ; Li, Xiu-Zhen 2 ; Seah, Stephen Y. K. 3 ; Peng, Bangzhu 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
2.Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada
3.Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada
4.Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Wuhan 430070, Peoples R China
5.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Genome Anal Lab, Guangdong Lab Lingnan Modern Agr,Shenzhen Branch,M, Shenzhen 518000, Peoples R China
关键词： Patulin; Biodegradation; Aldo-keto reductase; Molecular docking
期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）
ISSN： 0308-8146
年卷期： 2025 年 478 卷
页码：
收录情况： SCI
摘要： Patulin (PAT) is a hazardous mycotoxin that contaminates fruits and their products, causing significant economic losses. An aldo-keto reductase from Saccharomyces cerevisiae (ScAKR) was expressed in Escherichia coli in this study. The purified ScAKR converted PAT to E-ascladiol with NADPH as a cofactor. The ScAKR exhibited a strong degradation activity on PAT and the optimal degradation conditions were pH 7 and 37 degrees C. Molecular docking and site-specific mutagenesis indicated that the amino acids in ScAKR interacting with PAT aldehyde affected the degradation effect, and the mutation of Trp298 showed the most significant effect on the degradation rate. Furthermore, ScAKR also showed a strong degradation effect on 3-keto-deoxynivalenol, a metabolite of another mycotoxin, deoxynivalenol (DON). The findings offer new insights on the detoxification mechanism of PAT by S. cerevisiae and for the development and application of bioenzymes with broad-spectrum mycotoxin degradation properties.
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