Knocking-Out OsPDR7 Triggers Up-Regulation of OsZIP9 Expression and Enhances Zinc Accumulation in Rice
文献类型: 外文期刊
作者: Meng, Lu 1 ; Tang, Mingfeng 1 ; Zhu, Yuxing 1 ; Tan, Longtao 1 ;
作者机构: 1.Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chongqing Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Chongqing 401329, Peoples R China
关键词: OsPDR7; OsZIP9; zinc; metal accumulation; rice; ATP-binding cassette transporter
期刊名称:RICE SCIENCE ( 影响因子:4.8; 五年影响因子:5.5 )
ISSN: 1672-6308
年卷期: 2023 年 30 卷 1 期
页码:
收录情况: SCI
摘要: Zinc (Zn) is an essential trace mineral that is required for plant growth and development. A number of protein transporters, which are involved in Zn uptake, translocation and distribution, are finely regulated to maintain Zn homeostasis in plant. In this study, we functionally characterized an ATP-binding cassette (ABC) transporter gene, OsPDR7, which is involved in Zn homeostasis. OsPDR7 encodes a plasma membrane-localized protein that is expressed mainly in the exodermis and xylem in the rice root. ospdr7 mutants resulted in higher Zn accumulation compared with the wild type. Heterogeneous expression of OsPDR7 in a yeast mutant rescued the Zn-deficiency phenotype, implying transport activity of OsPDR7 to Zn in yeast. However, no ZIP genes except for OsZIP9 showed change in expression profile in the ospdr7 mutants, which suggested that OsPDR7 maintains cellular Zn homeostasis through regulating OsZIP9 expression. RNA-Seq analysis further revealed a set of differentially expressed genes between the wild type and ospdr7 mutants that allowed us to propose a possible OsPDR7-associated signaling network involving transporters, hormone responsive genes, and transcription factors. Our results revealed a novel transporter involved in the regulation of Zn homeostasis and will pave the way toward a better understanding of the fine-tuning of gene expression in the network of transporter genes.
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