Synergistic effect of high-pressure thermal sterilization and muramidase on Bacillus subtilis spores: alterations in intrasporal components, inner membrane permeability, and structural integrity
文献类型： 外文期刊
作者： Li, Jiajia 1 ; Xin, Weishan 1 ; Liu, Dunhua 1 ; Wang, Mengze 1 ; Liu, Mingan 1 ; Bi, Ke 3 ; Liu, Yue 1 ; Zhang, Zhong 1 ;
作者机构： 1.Ningxia Univ, Coll Food Sci & Engn, Yinchuan 750021, Peoples R China
2.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
3.Chinese Acad Agr Sci, Key Lab Agroprod Proc, Beijing 100193, Peoples R China
关键词： Bacillus subtilis; High-pressure thermal sterilization; Muramidase; Spore; Inner membrane; Structure integrity
期刊名称：ARCHIVES OF MICROBIOLOGY （ 影响因子：2.6； 五年影响因子：2.8 ）
ISSN： 0302-8933
年卷期： 2025 年 207 卷 6 期
页码：
收录情况： SCI
摘要： The sporicidal mechanism of high-pressure thermal sterilization (HPTS) combined with muramidase against Bacillus subtilis spores was investigated. Results demonstrated that HPTS at 600 MPa/75 degrees C with 0.3% muramidase achieved a 6.09 log reduction in Bacillus subtilis spores. The combined processing significantly increased the leakage of protein, nucleic acid, and dipicolinic acid, while significantly reducing Na+/K+-ATPase activity (P < 0.05). Scanning electron microscopy revealed notable morphological changes in spores after combined processing. A significant increase in propidium iodide (PI)-infiltrated spores indicated enhanced spore inner membrane permeability (P < 0.05). molecular composition analysis further showed disordered arrangement of fatty acid acyl chains, structural alterations in nucleic acids and proteins, and increased the peptidoglycan layer flexibility. These findings provided insights into the sporicidal mechanism of HPTS combined with muramidase.
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