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Study of the Stability and Anti-Inflammatory Activity of Paeonol-Oleanolic Acid Liposomes by Microfluidic Technology

文献类型： 外文期刊

作者： Ma, Xianzheng 1 ; Zhang, Hui 1 ; Luan, Jinkai 1 ; Tian, Mingfa 1 ; Zhang, Xiuxin 2 ; Sohail, Ammara 1 ; Liang, Dong 4 ; Liu, Jiguo 4 ; Tao, Fuzhan 4 ; Wang, Zheng 5 ; Wang, Daijie 1 ;

作者机构： 1.Qilu Univ Technol, Shandong Acad Sci, Biol Engn Technol Innovat Ctr Shandong Prov, Coll Pharm,Food Resources Dev & Hlth Prod Creat In, Heze 274000, Peoples R China

2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

3.Univ Okara, Dept Chem, Okara 56300, Pakistan

4.Heze Peony Ind Technol Res Inst, Heze 274000, Peoples R China

5.Qingdao Univ, Basic Med Coll, Dept Genet & Cell Biol, Qingdao 266071, Peoples R China

关键词： liposome; paeonol; oleanolic acid; cholesterol; anti-inflammatory activity

期刊名称：FOODS （ 影响因子：5.1； 五年影响因子：5.6 ）

ISSN：

年卷期： 2025 年 14 卷 12 期

页码：

收录情况： SCI

摘要： (1) Background: This study used a microfluidic approach to prepare paeonol (PAE) liposomes with oleanolic acid (PAE-ONLs) instead of cholesterol (PAE-CNLs), aiming to reduce cholesterol levels and enhance stability and anti-inflammatory activity. (2) Methods: The liposome formula was optimized, characterized, and tested for anti-inflammatory activities in zebrafish and RAW 264.7 macrophages, utilizing various stability and molecular interaction methods. (3) Results: The best PAE-ONL preparation conditions were 10.25 mg/mL of soy lecithin, 0.82 mg/mL of oleanolic acid, and 0.22% (wt%) of Tween 80, with an EE of 64.61 +/- 0.42%. TEM confirmed the uniform spherical morphology, and FTIR confirmed that oleanolic acid was incorporated into the liposomes. PAE-ONLs showed better stabilities than PAE-CNLs. Molecular interaction results revealed that PAE-ONLs achieved a greater energy reduction, reaching -85.07 kJ/mol vs. the -62.64 kJ/mol of PAE-CNLs, with stable hydrogen bonding interactions. PAE-ONLs significantly reduced inflammatory cell migration in zebrafish and decreased NO, TNF-alpha, IL-6, and IL-1 beta levels in LPS-induced RAW 264.7 macrophages at 20 mu g/mL. A network pharmacology analysis showed that oleanolic acid and paeonol interacted with 45 and 11 anti-inflammatory targets, respectively, and their combination in PAE-ONLs enhanced their anti-inflammatory coverage. (4) Conclusions: PAE-ONLs, utilizing oleanolic acid as a cholesterol substitute, exhibit enhanced stability and superior anti-inflammatory effects.

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