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Haplotype-resolved chromosomal-level genome assembly of Prunus mahaleb

文献类型: 外文期刊

作者: Fu, Chenlong 1 ; Li, Meng 2 ; Zhao, Zhuang 1 ; Yu, Lixia 3 ; Wang, Xianrong 2 ; Hou, Zhuangwei 4 ; Wang, Zefu 1 ;

作者机构: 1.Nanjing Forestry Univ, Coll Ecol & Environm, Coinnovat Ctr Sustainable Forestry Southern China, State Key Lab Tree Genet & Breeding, Nanjing 210037, Peoples R China

2.Nanjing Forestry Univ, Coll Life Sci, Nanjing 210037, Peoples R China

3.Shanghai Chenshan Bot Garden, Shanghai 201602, Peoples R China

4.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Key Lab Synth Biol, Guangdong Lab Lingnan Modern Agr,Shenzhen Branch,, Shenzhen, Peoples R China

期刊名称:SCIENTIFIC DATA ( 影响因子:6.9; 五年影响因子:8.7 )

ISSN:

年卷期: 2025 年 12 卷 1 期

页码:

收录情况: SCI

摘要: Prunus mahaleb is widely utilized as rootstock for cherries in Central Asia due to its exceptional resistance to environmental stressors. In this study, we generated a haplotype-resolved, chromosome-scale genome assembly for P. mahaleb using PacBio HiFi long reads and Hi-C technology. The resulting assembly comprises two haplotypes with sizes of 272.64 Mb (contig N50 = 26.60 Mb) and 271.76 Mb (contig N50 = 30.26 Mb), respectively. We identified 27,965 protein-coding genes (27,627 functionally annotated) and 27,931 protein-coding genes (27,607 functionally annotated) in the two haplotypes, respectively. Additionally, we annotated 145.64 Mb repetitive elements, 2,263 rRNAs, 430 tRNAs, and 780 other non-coding RNAs in haplotype A, and 144.04 Mb repetitive elements, 1,999 rRNAs, 442 tRNAs, and 734 other non-coding RNAs in haplotype B. Further analysis indicated that these haplotypes diverged approximately 2.67 million years ago. This high-quality genome assembly lays a solid groundwork for future initiatives in molecular breeding and functional genomics research for P. mahaleb.

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