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Molecular identification and related functional characterization of the FKBP52 gene in immunity of Locusta migratoria manilensis (Orthoptera: Oedipodidae)

文献类型： 外文期刊

作者： Wang, Menghong 1 ; Tian, Ye 2 ; Zhang, Neng 1 ; Nong, Xiangqun 1 ; Tu, Xiongbing 1 ; Zhang, Zehua 1 ; Huang, Yaxi 2 ; Wang, Yi 2 ; Zhuang, Ling 4 ; Cha, Gan 4 ; Liu, Tinghui 2 ; Wang, Guangjun 1 ;

作者机构： 1.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China

2.Hebei Agr Univ, Coll Plant Protect, Baoding, Hebei, Peoples R China

3.Minist Agr & Rural Affairs, Sci Observing & Expt Stn Pests Xilingol Rangeland, Beijing, Peoples R China

4.Bayannur Forestry & Grassland Dev Ctr, Bayannur, Peoples R China

关键词： FKBP52; Locusta migratoria manilensis; Metarhizium anisopliae; RNA interference

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.2； 五年影响因子：2.3 ）

ISSN： 0022-0493

年卷期： 2024 年 117 卷 3 期

页码：

收录情况： SCI

摘要： Metarhizium anisopliae is an important class of entomopathogenic fungi used for the biocontrol of insects, but its virulence is affected by insect immunity. We identified a novel FK506 binding protein gene that was differentially expressed between control and Metarhizium-treated Locusta migratoria manilensis. We hypothesized that this protein played an important role in Metarhizium infection of L. migratoria and could provide new insights for developing highly efficient entomopathogenic fungi. We, therefore, cloned the specific gene and obtained its purified protein. The gene was then named FKBP52, and its dsRNA (dsFKBP52) was synthesized and used for gene interference. Bioassay results showed that the mortality of L. migratoria treated with dsFKBP52 + Metarhizium was significantly lower than that of other treatments. Furthermore, immune-related genes (MyD88, Dorsal, Cactus, and Defensin) in L. migratoria treated with dsFKBP52 + Metarhizium showed significant upregulation compared to that treated with Metarhizium only. However, the activities of peroxidase (POD), superoxide dismutase (SOD), and calcineurin (CaN) showed fluctuations. These results suggest that the FKBP52 gene may play a crucial role in the innate immunity of L. migratoria. The effect of its silencing indicated that this immunity-related protein might be a potential target for insect biocontrol.

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