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Engineering DMNT emission in cotton enhances direct and indirect defense against mirid bugs

文献类型： 外文期刊

作者： Teng, Dong 1 ; Liu, Danfeng 2 ; Khashaveh, Adel 1 ; Lv, Beibei 1 ; Sun, Peiyao 1 ; Geng, Ting 5 ; Cui, Hongzhi 6 ; Wang, Yi 2 ; Zhang, Yongjun 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.Yunnan Univ, Inst Biodivers, Sch Ecol & Environm Sci, Yunnan Key Lab Plant Reprod Adaptat & Evolutionary, Kunming 650504, Peoples R China

3.Yunnan Univ, Inst Biodivers, Ctr Invas Biol, Sch Ecol & Environm Sci, Kunming 650504, Peoples R China

4.Shanxi Agr Univ, Inst Cotton Res, Yuncheng 044000, Peoples R China

5.Natl Plant Protect Sci Observat & Expt Stn, Langfang 065000, Peoples R China

6.Biocentury Transgene China Co Ltd, Shenzhen 518117, Peoples R China

关键词： Plant defense; DMNT biosynthesis; CRISPR/Cas9; Overexpression; Mirid bug; Ecological trap

期刊名称：JOURNAL OF ADVANCED RESEARCH （ 影响因子：13.0； 五年影响因子：11.6 ）

ISSN： 2090-1232

年卷期： 2025 年 71 卷

页码：

收录情况： SCI

摘要： Introduction: As an important herbivore-induced plant volatile, (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) is known for its defensive role against multiple insect pests, including attracting natural enemies. A terpene synthase (GhTPS14) and two cytochrome P450 (GhCYP82L1, GhCYP82L2) enzymes are involved in the de novo synthesis of DMNT in cotton. We conducted a study to test the potential of manipulating DMNT-synthesizing enzymes to enhance plant resistance to insects. Objectives: To manipulate DMNT emissions in cotton and generate cotton lines with increased resistance to mirid bug Apolygus lucorum. Methods: Biosynthesis and emission of DMNT by cotton plants were altered using CRISPR/Cas9 and over-expression approaches. Dynamic headspace sampling and GC-MS analysis were used to collect, identify and quantify volatiles. The attractiveness and suitability of cotton lines against mirid bug and its parasitoid Peristenus spretus were evaluated through various assays. Results: No DMNT emission was detected in knockout CAS-L1L2 line, where both GhCYP82L1 and GhCYP82L2 were knocked out. In contrast, gene-overexpressed lines released higher amounts of DMNT when infested by A. lucorum. At the flowering stage, L114 (co-overexpressing GhCYP82L1 and GhTPS14) emitted 10-15-fold higher amounts than controls. DMNT emission in overexpressed transgenic lines could be triggered by methyl jasmonate (MeJA) treatment. Apolygus lucorum and its parasitoid were far less attracted to the double edited CAS-L1L2 plants, however, co-overexpressed line L114 significantly attracted bugs and female wasps. A high dose of DMNT, comparable to the emission of L114, significantly inhibited the growth of A. lucorum, and further resulted in higher mortalities. Conclusion: Turning down DMNT emission attenuated the behavioral preferences of A. lucorum to cotton. Genetically modified cotton plants with elevated DMNT emission not only recruited parasitoids to enhance indirect defense, but also formed an ecological trap to kill the bugs. Therefore, manipulation of DMNT biosynthesis and emission in plants presents a promising strategy for controlling mirid bugs. (c) 2024 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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