文献类型： 外文期刊

作者： Liu, Xu ¹ ; Park, Helen ¹ ; Ackermann, Yannic Sebastian ⁶ ; Averous, Luc ⁷ ; Ballerstedt, Hendrik ⁸ ; Besenmatter, Werner ⁹ ; Blazquez, Blas ¹⁰ ; Bornscheuer, Uwe T. ¹¹ ; Branson, Yannick ¹¹ ; Casey, William ¹² ; de Lorenzo, Victor ¹³ ; Dong, Weiliang ¹⁴ ; Floehr, Tilman ¹⁵ ; Godoy, Manuel S. ¹⁶ ; Ji, Yu ¹⁷ ; Jupke, Andreas ¹⁸ ; Klankermayer, Juergen ¹⁹ ; San Leon, David ¹⁰ ; Liu, Luo ²⁰ ; Liu, Xianrui ²⁰ ; Liu, Yizhi ²⁰ ; Manoli, Maria T. ¹⁶ ; Martinez-Garcia, Esteban ¹³ ; Naranciz, Tanja ²¹ ; Nogales, Juan ¹⁰ ; O'Connor, Kevin ²¹ ; Osterthun, Ole ¹⁹ ; Perrin, Remi ²² ; Prieto, M. Auxiliadora ¹ ; Pollet, Eric ¹ ; Sarbu, Alexandru ¹ ; Schwaneberg, Ulrich ¹⁷ ; Su, Haijia ²⁰ ; Tang, Zequn ²⁰ ; Tiso, Till ⁸ ; Wang, Zishuai ²⁰ ; Wei, Ren ¹¹ ; Welsing, Gina ⁸ ; Wierckx, Nick ⁶ ; Wolter, Birger ⁸ ; Xiao, Gang ¹ ; Xing, Jianmin ²³ ; Zhao, Yilin ²⁰ ; Zhou, Jie ¹⁴ ; Tan, Tianwei ²⁴ ; Blank, Lars M. ⁸ ; Jiang, Min ¹⁴ ; Chen, Guo-Qiang ¹ ;

作者机构： 1.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China

2.PhaBuilder Biotechnol Co Ltd, Beijing 101309, Peoples R China

3.Chinese Acad Agr Sci, State Key Lab Anim Nutr & Feeding, Inst Anim Sci, Beijing 100193, Peoples R China

4.Univ Manchester, Manchester Inst Biotechnol, BBSRC Synthet Biol Res Ctr,SYNBIOCHEM, Sch Nat Sci,EPSRC/BBSRC Future Biomfg Res Hub, Manchester M1 7DN, England

5.Univ Manchester, Sch Nat Sci, Dept Chem, Manchester M1 7DN, England

6.Forschungszentrum Julich, Inst Bio & Geosci IBG 1 Biotechnol, Julich, Germany

7.Univ Strasbourg, BioTeam, ICPEES ECPM, UMR 7515,CNRS, 25 Rue Becquerel, F-67087 Strasbourg 2, France

8.Rhein Westfal TH Aachen, Inst Appl Microbiol iAMB, Aachen Biol & Biotechnol ABBt, Worringerweg 1, D-52074 Aachen, Germany

9.AB Enzymes GmbH, Feldbergstr 78, D-64293 Darmstadt, Germany

10.CSIC, Ctr Nacl Biotecnol, Dept Syst Biol, Syst Biotechnol Grp, Madrid, Spain

11.Univ Greifswald, Inst Biochem, Dept Biotechnol & Enzyme Catalysis, Felix Hausdorff Str 4, D-17487 Greifswald, Germany

12.Univ Coll Dublin, Bioplastech Ltd, Nova UCD, Belfield Innovat Pk, Dublin, Ireland

13.CSIC, Ctr Nacl Biotecnol, Dept Syst Biol, Environm Synthet Biol Lab, Madrid, Spain

14.Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Peoples R China

15.Everwave GmbH, Struverweg 116, D-52070 Aachen, Germany

16.Spanish Natl Res Council CIB CSIC, Biol Res Ctr Margarita Salas, Polymer Biotechnol Lab, Madrid, Spain

17.Rhein Westfal TH Aachen, Inst Biotechnol BIOTEC, Aachen Biol & Biotechnol ABBt, Worringerweg 3, D-52074 Aachen, Germany

18.Rhein Westfal TH Aachen, Fluid Proc Engn, Aachen Proc Technol AVT, Forckenbeckstr 51, D-52074 Aachen, Germany

19.Rhein Westfal TH Aachen, Inst Tech & Macromol Chem ITMC, Worringerweg 2, D-52074 Aachen, Germany

20.Beijing Univ Chem Technol, Coll Life Sci & Technol, State Key Lab Chem Resource Engn, Beijing Key Lab Bioproc, Beijing, Peoples R China

21.Univ Coll Dublin, BiOrb Bioecon SFI Res Ctr, Sch Biomol & Biomed Sci, Dublin, Ireland

22.SOPREMA, Direct R&D, 14 Rue St Nazaire, F-67100 Strasbourg, France

23.Chinese Acad Sci, Inst Proc Engn IPE, State Key Lab Biochem Engn, 1 North 2nd St, Beijing 100190, Peoples R China

24.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China

25.State Key Lab Green Biomfg, Beijing, Peoples R China

关键词： Biodegradable materials; Plastic degradation; Enzymatic recycling; Polyhydroxyalkanoate (PHA); Polyethylene terephthalate (PET); Microbial depolymerization; Biocatalytic plastic degradation; Circular economy

期刊名称：BIOTECHNOLOGY ADVANCES （ 影响因子：12.5； 五年影响因子：15.7 ）

ISSN： 0734-9750

年卷期： 2025 年 81 卷

页码：

收录情况： SCI

摘要： The persistent demand for plastic commodities, inadequate recycling infrastructure, and pervasive environmental contamination due to plastic waste present a formidable global challenge. Recycling, degradation and upcycling are the three most important ways to solve the problem of plastic pollution. Sequential enzymatic and microbial degradation of mechanically and chemically pre-treated plastic waste can be orchestrated, followed by microbial conversion into value-added chemicals and polymers through mixed culture systems. Furthermore, plastics-degrading enzymes can be optimized through protein engineering to enhance their specific binding capacities, stability, and catalytic efficiency across a broad spectrum of polymer substrates under challenging high salinity and temperature conditions. Also, the production and formulation of enzyme mixtures can be finetuned to suit specific waste compositions, facilitating their effective deployment both in vitro, in vivo and in combination with chemical technologies. Here, we emphasized the comprehensive strategy leveraging microbial processes to transform mixed plastics of fossil-derived polymers such as PP, PE, PU, PET, and PS, most notably polyesters, in conjunction with potential biodegradable alternatives such as PLA and PHA. Any residual material resistant to enzymatic degradation can be reintroduced into the process loop following appropriate physicochemical treatment.