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Functional Analysis of β-Tubulins in Ustilaginoidea virens and Their Contribution to Carbendazim Resistance

文献类型： 外文期刊

作者： Song, Jiehui 1 ; Wang, Ying 1 ; Wang, Zhiying 1 ; Xing, Zhipeng 1 ; Xu, Qiang 1 ; Li, Guohui 1 ; Chen, Shuning 3 ; Xu, Ke 1 ;

作者机构： 1.Yangzhou Univ, Agr Coll, Jiangsu Key Lab Crop Genet & Physiol, Yangzhou 225009, Jiangsu, Peoples R China

2.Yangzhou Univ, Agr Coll, Coinnovat Ctr Modern Prod Technol Grain Crops, Yangzhou 225009, Jiangsu, Peoples R China

3.Chinese Acad Agr Sci, Inst Plant Protect, Key Lab Pesticides Evaluat, Minist Agr, Beijing 100193, Peoples R China

关键词： beta-tubulin; carbendazim resistance; molecularmechanism; Ustilaginoidea virens

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2025 年

页码：

收录情况： SCI

摘要： The rice false smut disease, caused by Ustilaginoidea virens, represents a significant fungal threat to rice production. Our investigation aimed to elucidate the functional roles of two beta-tubulin paralogs (Uv beta 1tub and Uv beta 2tub) in fungal biology and chemical adaptation. Through targeted gene editing, it was demonstrated that both Uv beta 1tub and Uv beta 2tub were essential for vegetative growth, cellular morphology maintenance, conidiation, stress adaptation, and virulence in U. virens. The contrasting resistance phenotypes of Delta Uv beta 1tub (enhanced carbendazim resistance) versus Delta Uv beta 2tub (hypersensitivity) demonstrate that Uv beta 1tub serves as the primary target of carbendazim. The critical determinant of this differential sensitivity was the natural amino acid divergence at position 198, specifically alanine (A198) in Uv beta 2tub versus glutamic acid (E198) in Uv beta 1tub. Site-directed mutagenesis confirmed this molecular mechanism: the A198E substitution in Uv beta 2tub abolished its intrinsic carbendazim insensitivity, while the reciprocal E198A mutation in Uv beta 1tub conferred carbendazim resistance. Expression analysis revealed stable transcript levels of both beta-tubulin genes during vegetative growth and infection; however, deletion of either gene triggered compensatory upregulation of its paralog. Collectively, our results elucidate the functional significance of beta-tubulin isotypes and their evolutionarily conserved structural motifs in U. virens, thereby advancing the mechanistic understanding of beta-tubulin-mediated sensitivity to carbendazim in phytopathogenic fungi.

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