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A programmable nucleic acid fluorescence biosensor based on the BstNI endonuclease for detection of the influenza A (H1N1) virus

文献类型： 外文期刊

作者： Sa, Qila 1 ; Sun, He 2 ; Bu, Shengjun 2 ; Xu, Yao 2 ; Zhang, Zebin 1 ; Li, Chenxi 1 ; Wang, Longtao 1 ; Wan, Jiayu 2 ;

作者机构： 1.Jilin Agr Univ, Coll Vet Med, Changchun 130118, Peoples R China

2.Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun 130122, Peoples R China

关键词： BstNI endonuclease; Primer exchange reaction; Fluorescent biosensor

期刊名称：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY （ 影响因子：4.6； 五年影响因子：4.3 ）

ISSN： 1386-1425

年卷期： 2025 年 335 卷

页码：

收录情况： SCI

摘要： Timely and accurate diagnosis of influenza virus is essential to prevent the spread of disease and to select an appropriate treatment strategy. Here, we report the development of a novel fluorescence biosensor for detection of the H1N1 virus based on the BstNI endonuclease, dually-blocked RNA strands (S), and FAM-ssDNA-Q reporter (R) strands. The S strand contains a short (5 nt) sequence for BstNI recognition and sequences on both sides of the cutting site, which are closed by two locking strands. The R strand is complementary to the intermediate sequence of the S strand, including the BstNI cutting site domain and partial adjacent sequences. Only the presence of two specific RNA fragments of the target influenza virus can fully de-block the S strands, which then hybridize with the R strands, followed by cleavage via BstNI-catalyzed hydrolysis, thereby generating the fluorescence signal. The biosensor was sensitive to the H1N1 virus at 100 fM and was highly specific to the target sequence. The proposed biosensor provides a convenient method for reliable detection of the H1N1 virus.

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