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Lateral flow biosensor development for the visual identification of H1N1 virus based on primer extension nucleic acid isothermal amplification and M13mp18 single-stranded DNA

文献类型： 外文期刊

作者： Wang, Lingling 1 ; Bu, Shengjun 2 ; Meng, Heyuan 2 ; Zhang, Xiaoying 1 ; Zhou, Hongyu 2 ; Xu, Chuanna 3 ; Hao, Zhuo 2 ; Su, Yuchun 1 ; Wan, Jiayu 2 ;

作者机构： 1.Jilin Agr Univ, Coll Life Sci, Changchun 130118, Peoples R China

2.Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun 130122, Peoples R China

3.Changchun Univ Sci & Technol, Sch Life Sci & Technol, Changchun 130022, Peoples R China

关键词： Influenza virus; M13mp18 ssDNA; Lateral flow assay; Isothermal amplification

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.3； 五年影响因子：4.9 ）

ISSN： 0026-3672

年卷期： 2025 年 192 卷 3 期

页码：

收录情况： SCI

摘要： A lateral flow biosensor was developed based on newly established primer extension nucleic acid isothermal amplification (PEIA) and M13mp18 single-stranded DNA (ssDNA) to visually identify the H1N1 virus. The PEIA process was primer based on a template to continuously extend multiple repeat sequences, using DNA polymerase, to form long ssDNAs. M13mp18 ssDNA was then coupled to multiple long ssDNAs from PEIA and functionalized in a signal tag DNA nanostructure (STDN) for the lateral flow biosensor. In the presence of target H1N1-RNA, the sandwich structure capture probe/H1N1-RNA/STDN is fabricated caught in the lateral flow biosensor test zone, and appears red. Our biosensor successfully identified H1N1 virus RNA over a broad range of concentrations (10-106 pM) with a minimum visual detection limit of 10 pM. In conclusion, our biosensor may be applied to point-of-care diagnostics in low-resource environments with non-specialist staff.

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