Recent Advances of Tannase: Production, Characterization, Purification, and Application in the Tea Industry
文献类型： 外文期刊
作者： Tang, Zhanhui 1 ; Shi, Liyu 1 ; Liang, Shuang 2 ; Yin, Junfeng 2 ; Dong, Wenjiang 3 ; Zou, Chun 2 ; Xu, Yongquan 4 ;
作者机构： 1.Zhejiang Wanli Univ, Coll Biol & Environm Sci, Ningbo 315100, Peoples R China
2.Chinese Acad Agr Sci, Tea Res Inst, Key Lab Biol, Minist Agr & Rural Affairs, 9 South Meiling Rd, Hangzhou 310008, Peoples R China
3.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning 571533, Peoples R China
4.Natl Engn Res Ctr Tea Proc, Hangzhou 310008, Peoples R China
关键词： Tannase; fermentation; enzymatic characterization; purification; application
期刊名称：FOODS （ 影响因子：5.1； 五年影响因子：5.6 ）
ISSN：
年卷期： 2025 年 14 卷 1 期
页码：
收录情况： SCI
摘要： Tannase, as a type of tannin-degrading enzyme, can catalyze the hydrolysis of ester and depside bonds in gallotannins, thereby releasing gallic acid and glucose. Based on this reaction mechanism, Tannase can effectively improve the problems of bitter taste, weak aroma, and tea cheese in tea infusion, and is therefore widely used in the tea industry. However, due to high production costs, difficulties in purification and recovery, and insufficient understanding of Tannase properties, the large-scale application of Tannase is severely limited. Therefore, the sources of Tannase and the effects of fermentation temperature, pH, stirring speed, time, carbon, and nitrogen sources on the preparation of Tannase are described in this study. The advantages and disadvantages of various methods for measuring Tannase activity and their enzymatic characterization are summarized, and the concentration and purification methods of Tannase are emphasized. Finally, the application of Tannase to reduce the formation of tea precipitate, enhance antioxidant capacity, increase the extraction rate of active ingredients, and improve the flavor of the tea infusion is described. This study systematically reviews the production, characterization, purification, and application of Tannase to provide a reference for further research and application of Tannase.
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