RNAi-based silencing of proteasome 20S subunit alpha 2 affected the survival and development of Henosepilachna vigintioctopunctata
文献类型： 外文期刊
作者： Liu, Junna 1 ; Guo, Mujuan 1 ; Nanda, Satyabrata 2 ; Li, Zhaoyang 1 ; Zhou, Xuguo 3 ; Zhang, Youjun 4 ; Yang, Chunxiao 5 ; Pan, Huipeng 1 ;
作者机构： 1.South China Agr Univ, Coll Plant Protect, Natl Key Lab Green Pesticide, Guangzhou 510642, Peoples R China
2.Centurion Univ Technol & Management, MS Swaminathan Sch Agr, Paralakhemundi 761200, India
3.Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA
4.Chinese Acad Agr Sci, Dept Plant Protect, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
5.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Peoples R China
关键词： Henosepilachna vigintioctopunctata; Dietary RNAi; Feeding disorders; Pros alpha 2; Biopesticide
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）
ISSN： 0048-3575
年卷期： 2023 年 195 卷
页码：
收录情况： SCI
摘要： Henosepilachna vigintioctopunctata is a notorious pest of solanaceous plants in Asia, which is mainly managed by chemical pesticides. RNA interference (RNAi) technique is considered to be a promising and effective alternative for pest control. In this study, we selected the proteasome 20S subunit alpha 2 (Pros alpha 2) gene, a cellular protein involved in many proteins regulatory processes, to explore the RNAi efficiency in H. vigintioctopunctata. The obtained results confirmed the significant lethal effects of HvPros alpha 2 silencing on the H. vigintioctopunctata 1st instar larvae at concentrations of 100, 50, and 5 ng/mu L. Ingestion of the bacterially expressed dsHvPros alpha 2 caused high mortality in both larvae and adults. Moreover, silencing of HvPros alpha 2 resulted in feeding disorders, growth delay, and abnormal intestinal development of the larvae. Overall, HvPros alpha 2 acts as an important regulator for the growth and development of H. vigintioctopunctata, and can serve as a candidate target gene for the RNAibased control of H. vigintioctopunctata.
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