您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Cooperative application of transcriptomics and ceRNA hypothesis: LncRNA-107052630/miR-205a/G0S2 crosstalk is involved in ammonia-induced intestinal apoptotic injury in chicken

文献类型： 外文期刊

作者： Wang Shengchen 1 ; Wang Wei 1 ; Li Xiaojing 1 ; Zhao Xia 1 ; Wang Yue 1 ; Zhang Hongfu 2 ; Xu Shiwen 1 ;

作者机构： 1.Northeast Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China

2.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China

3.Northeast Agr Univ, Coll Vet Med, Dept Heilongjiang Common Anim Dis Prevent & Treat, Key Lab Prov Educ, Harbin 150030, Peoples R China

关键词： Ammonia; jejunum; Apoptosis; ceRNA; Transcriptome

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：10.588； 五年影响因子：10.129 ）

ISSN： 0304-3894

年卷期： 2020 年 396 卷

页码：

收录情况： SCI

摘要： Ammonia (NH3), as a harmful gas from agricultural production, plays an important role in air pollution, such as haze. Although numerous researchers have paid attention to health damage through NH3 inhalation, the exhaustive mechanism of NH3 induced intestinal toxicity remains unclear. A genes crosstalk named competing endogenous RNAs (ceRNA) can explain many regulatory manners from the molecular perspective. However, few studies have attempted to interpret the injury mechanism of air pollutants to the organism via ceRNA theory. Here, we thoroughly investigated the lncRNA-associated-ceRNA mechanism in jejunum samples from a 42-days-old NH3-exposed chicken model through deep RNA sequencing. We observed the occurrence of apoptosis in jejunum, obtained 46 significantly dysregulated lncRNAs and 30 dysregulated miRNAs, and then constructed lncRNA-associated-ceRNA networks in jejunum. Importantly, a network regulating G0S2 in NH3-induced apoptosis was discovered. Research results showed that G0S2 was upregulated in jejunum of NH3-exposed group and was associated with activation of the mitochondrial apoptosis pathway. G0S2 antagonized the anti-apoptotic effect of Bcl2, which could be reversed by miR-205a. Meanwhile, lncRNA-107052630 acted as ceRNA to affect G0S2 function. These data provide new insight for revealing the biological effect of NH3 toxicity, as well as the environmental research.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台